Patterns & Phenotypes
The dynamic gene expression patterns of transcription factors constituting the sea urchin aboral ectoderm gene regulatory network
Article first published online: 22 DEC 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 240, Issue 1, pages 250–260, January 2011
How to Cite
Chen, J.-H., Luo, Y.-J. and Su, Y.-H. (2011), The dynamic gene expression patterns of transcription factors constituting the sea urchin aboral ectoderm gene regulatory network. Dev. Dyn., 240: 250–260. doi: 10.1002/dvdy.22514
- Issue published online: 22 DEC 2010
- Article first published online: 22 DEC 2010
- Manuscript Accepted: 1 NOV 2010
- ICOB, Academia Sinica, Taiwan
- gene regulatory network;
- aboral ectoderm;
- sea urchin
The temporal and spatial expression patterns of regulatory genes are required for building a gene regulatory network (GRN). The current ectoderm GRN model for the sea urchin embryo includes pregastrular specification functions in the oral (OE) and aboral ectoderm (AE). Unlike the OE, which is resolved into several subdomains, the AE is considered a simpler territory due to the lack of detailed gene expression studies in this territory. Here, we perform temporal and spatial gene expression studies on the eight transcription factor genes constituting the AE GRN. Based on the differential gene expression patterns, we conclude that the AE contains at least three subdomains at the mesenchyme blastula stage. We also performed immunostaining for pSmad1/5/8 to monitor the activation of the BMP signaling pathway. The dynamic changes in the expression patterns of these transcription factor genes and the nuclearization of pSmad1/5/8 may provide a foundation for resolving the AE GRN. Developmental Dynamics, 2011. © 2010 Wiley-Liss, Inc.