Patterns & Phenotypes
Identification and expression patterns of members of the protease-activated receptor (par) gene family during zebrafish development
Article first published online: 22 DEC 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 240, Issue 1, pages 278–287, January 2011
How to Cite
Xu, H., Echemendia, N., Chen, S. and Lin, F. (2011), Identification and expression patterns of members of the protease-activated receptor (par) gene family during zebrafish development. Dev. Dyn., 240: 278–287. doi: 10.1002/dvdy.22517
- Issue published online: 22 DEC 2010
- Article first published online: 22 DEC 2010
- Manuscript Accepted: 6 NOV 2010
- NIH/NCRR. Grant Number: K99R00RR024119
- NIH/NIGMS. Grant Number: R01GM77770
- protease-activated receptors (PARs);
- expression pattern;
Protease-activated receptors (PARs) play critical roles in hemostasis in vertebrates including zebrafish. However, the zebrafish gene classification appears to be complex, and the expression patterns of par genes are not established. Based on analyses of genomic organization, phylogenetics, protein primary structure, and protein internalization, we report the identification of four zebrafish PARs: par1, par2a, par2b, and par3. This classification differs from one reported previously. We also show that these genes have distinct spatiotemporal expression profiles in embryos and larvae, with par1, par2a, and par2b expressed maternally and ubiquitously during gastrula stages and their expression patterns refined at later stages, and par3 expressed only in 3-day-old larvae. Notably, the expression patterns of zebrafish par1 and par2b resemble those of their mammalian counterparts, suggesting that receptor function is conserved among vertebrates. This conservation is supported by our findings that Par1 and Par2b are internalized following exposure to thrombin and trypsin, respectively. Developmental Dynamics, 2011. © 2010 Wiley-Liss, Inc.