Long form of latent TGF-β binding protein 1 (Ltbp1L) regulates cardiac valve development
Article first published online: 22 DEC 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 240, Issue 1, pages 176–187, January 2011
How to Cite
Todorovic, V., Finnegan, E., Freyer, L., Zilberberg, L., Ota, M. and Rifkin, D. B. (2011), Long form of latent TGF-β binding protein 1 (Ltbp1L) regulates cardiac valve development. Dev. Dyn., 240: 176–187. doi: 10.1002/dvdy.22521
- Issue published online: 22 DEC 2010
- Article first published online: 22 DEC 2010
- Manuscript Accepted: 11 NOV 2010
- NIH. Grant Numbers: R01CA34282, P01AR049698
Transforming Growth Factor β (TGF-β) is crucial for valve development and homeostasis. The long form of Latent TGF-β binding protein 1 (LTBP1L) covalently binds all TGF-β isoforms and regulates their bioavailability. Ltbp1L expression analysis during valvulogenesis revealed two patterns of Ltbp1L production: an early one (E9.5–11.5) associated with endothelial-to-mesenchymal transformation (EMT); and a late one (E12.5 to birth) contemporaneous with valve remodeling. Similarly, histological analysis of Ltbp1L−/− developing valves identified two different pathologies: generation of hypoplastic endocardial cushions in early valvulogenesis, followed by development of hyperplastic valves in late valvulogenesis. Ltbp1L promotes valve EMT, as Ltbp1L absence yields hypoplastic endocardial cushions in vivo and attenuated EMT in vitro. Ltbp1L−/− valve hyperplasia in late valvuogenesis represents a consequence of prolonged EMT. We demonstrate that Ltbp1L is a major regulator of Tgf-β activity during valvulogenesis since its absence results in a perturbed Tgf-β pathway that causes all Ltbp1L−/− valvular defects. Developmental Dynamics, 2011. © 2010 Wiley-Liss, Inc.