Noncanonical notch function in motor axon guidance is mediated by Rac GTPase and the GEF1 domain of trio
Article first published online: 3 JAN 2011
Copyright © 2011 Wiley-Liss, Inc.
Volume 240, Issue 2, pages 324–332, February 2011
How to Cite
Song, J. K. and Giniger, E. (2011), Noncanonical notch function in motor axon guidance is mediated by Rac GTPase and the GEF1 domain of trio. Dev. Dyn., 240: 324–332. doi: 10.1002/dvdy.22525
- Issue published online: 18 JAN 2011
- Article first published online: 3 JAN 2011
- Manuscript Accepted: 23 NOV 2010
- NIH. Grant Number: NINDS: Z01NS003013
- axon guidance;
The receptor Notch interacts with the Abl tyrosine kinase signaling pathway to control axon growth and guidance in Drosophila motor neurons. In part, this is mediated by binding to Trio, a guanine nucleotide exchange factor (GEF) for Rho GTPases. We show here that one of the two GEF domains of Trio, the Rac-specific GEF1, is essential for Trio-dependent motor axon guidance and for the genetic suppression of Notch function in motor axon patterning, but the Rho-specific GEF2 domain is not. Consistent with this, we show that Rac, and not Rho1 or Cdc42, interacts genetically with Notch in a manner indistinguishable from that of bona fide Abl signaling components. We infer, therefore, that Rac is a key component of Abl signaling in Drosophila motor axons, and specifically that it is the crucial Rho GTPase in “noncanonical” Notch/Abl signaling. Developmental Dynamics 240:324–332, 2011. © 2011 Wiley-Liss, Inc.