Translocon-associated protein subunit Trap-γ/Ssr3 is required for vascular network formation in the mouse placenta

Authors

  • Yasuka L. Yamaguchi,

    1. Department of Kidney Development, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Kumamoto, Japan
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    • Yasuka L. Yamaguchi and Satomi S. Tanaka contributed equally to this work.

  • Satomi S. Tanaka,

    Corresponding author
    1. Department of Kidney Development, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Kumamoto, Japan
    • Department of Kidney Development, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Kumamoto, Japan
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    • Yasuka L. Yamaguchi and Satomi S. Tanaka contributed equally to this work.

  • Naoko Oshima,

    1. Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Kobe, Japan
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  • Hiroshi Kiyonari,

    1. Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Kobe, Japan
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  • Makoto Asashima,

    1. Research Center for Stem Cell Engineering (SCRC), National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan
    2. Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan
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  • Ryuichi Nishinakamura

    Corresponding author
    1. Department of Kidney Development, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Kumamoto, Japan
    2. Global COE “Cell Fate Regulation Research and Education Unit,” Kumamoto University, Kumamoto, Japan
    • Department of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan
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Abstract

The translocon-associated protein (TRAP, also termed the signal sequence receptor) complex is required for the efficient translocation of secretory and membrane proteins in the endoplasmic reticulum, and is also involved in the endoplasmic reticulum stress-mediated unfolded protein response pathway. To investigate the roles of Trap-γ, a TRAP complex subunit, we generated Trap-γ knockout mice and found that mutant pups died soon after birth because of retarded embryonic organ growth, especially in the lung. The mutant placentae showed severe vascular network malformation in the labyrinth and significant reductions in blood space areas, which had an adverse effect on intrauterine embryonic growth. Placental malformation was already found by the mid-gestation-stage mutant placenta, with poor vascular endothelial cell proliferation in the chorionic plate region and increased apoptotic cell death in the labyrinth. Thus, Trap-γ appears to be required for vascular network formation in murine placental development. Developmental Dynamics 240:394–403, 2011. © 2011 Wiley-Liss, Inc.

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