Disclosure of Potential Conflicts of Interest The authors have no conflicts of interest to declare.
Patterns & Phenotypes
Stem cell marker prominin-1 regulates branching morphogenesis, but not regenerative capacity, in the mammary gland†
Version of Record online: 11 JAN 2011
Copyright © 2010 Wiley-Liss, Inc.
Special Issue: Special Focus on Endoderm
Volume 240, Issue 3, pages 674–681, March 2011
How to Cite
Anderson, L. H., Boulanger, C. A., Smith, G. H., Carmeliet, P. and Watson, C. J. (2011), Stem cell marker prominin-1 regulates branching morphogenesis, but not regenerative capacity, in the mammary gland. Dev. Dyn., 240: 674–681. doi: 10.1002/dvdy.22539
- Issue online: 17 FEB 2011
- Version of Record online: 11 JAN 2011
- Manuscript Accepted: 8 DEC 2010
- Intramural Research Program of the Center for Cancer Research (NCI, NIH)
- Flemish Government. Grant Number: FWO G.0209.07
- Flemish Government; Methusalem funding
Additional Supporting Information may be found in the online version of this article.
|DVDY_22539_sm_suppfig1.tif||1306K||Supporting Figure 1 Prom1−/− tissue responds normally to bFGF Organoids from Prom1+/+ and Prom1−/− mammary tissue grown in Matrigel for 7 days, with or without bFGF supplementation. Scale bar = 100 μm.|
|DVDY_22539_sm_suppfig2.tif||1282K||Supporting Figure 2 Prom1 loss does not cause abnormal cellular composition or polarity in mammary ducts. Tissue sections from Prom1+/+ and Prom1−/− mice labeled with antibodies against (a) cytokeratin 18 (K18, red) and cytokeratin 14 (K14, green), and (b) ZO1 (red) and K14 (green), showing normal distribution in the mammary ducts of both genotypes. Scale bars = 50 μm.|
|DVDY_22539_sm_suppfig3.tif||311K||Supporting Figure 3 Prom1 expression decreases during pregnancy. Immunofluorescence of Prom1 (red) expression in virgin mammary tissue and throughout pregnancy. Scale bars = 50 μm.|
|DVDY_22539_sm_suppfig4.tif||879K||Supporting Figure 4 Prom1 loss is associated with increased luminal cell proliferation in vitro. a: Individual colonies established in 2D culture, labeled for K18 (red) and smooth muscle actin (SMA, green). Scale bars = 100 μm. b: Western blot analysis of ERK activation (phosphorylation) in mammary tissue from 4–5-week-old Prom1+/+ and Prom1−/− mice, with a β-actin loading control. c: RT-PCR analysis of cyclin D1 (Ccnd1) cDNA levels in mammary tissue from 4–5-week-old Prom1+/+ and Prom1−/− mice, with a Gapdh control. d: Quantification of the area of individual colonies from Prom1+/+ and Prom1−/− cells collected from virgin (n=2) and parous (n=4) mice. *P < 0.05, **P < 0.01. Scale bars = SD.|
|DVDY_22539_sm_suppfig5.tif||461K||Supporting Figure 5 Prom1 loss does not affect cilia distribution. Immunofluorescence labeling for cilia (acetylated α-tubulin, red) in (a) ovarian follicles and (b) mammary ducts, from Prom1+/+ and Prom1−/− mice. Scale bars = (a) 25 μm, (b) 50 μm. O, oocyte; fc, follicle cells.|
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