Autotaxin is required for the cranial neural tube closure and establishment of the midbrain–hindbrain boundary during mouse development

Authors

  • Seiichi Koike,

    1. Department of Molecular Neurobiology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan
    Current affiliation:
    1. Seiichi Koike's present address is Department of Neurobiology, Max Planck Institute of Biophysical Chemistry, Am Faßberg 11, 37077 Göttingen, Germany
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  • Yoshifumi Yutoh,

    1. Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, Tokushima, Japan
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  • Kazuko Keino-Masu,

    1. Department of Molecular Neurobiology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan
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  • Sumihare Noji,

    1. Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, Tokushima, Japan
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  • Masayuki Masu,

    Corresponding author
    1. Department of Molecular Neurobiology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan
    • Department of Molecular Neurobiology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8577, Japan
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  • Hideyo Ohuchi

    Corresponding author
    1. Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, Tokushima, Japan
    • Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, Tokushima, 770-8506, Japan
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Abstract

Autotaxin (ATX) is a lysophospholipid-generating exoenzyme expressed in embryonic and adult neural tissues. We previously showed that ATX is expressed in the neural organizing centers, anterior head process, and midbrain-hindbrain boundary (MHB). To elucidate the role of ATX during neural development, here we examined the neural phenotypes of ATX-deficient mice. Expression analysis of neural marker genes revealed that lateral expansion of the rostral forebrain is reduced and establishment of the MHB is compromised as early as the late headfold stage in ATX mutant embryos. Moreover, ATX mutant embryos fail to complete cranial neural tube closure. These results indicate that ATX is essential for cranial neurulation and MHB establishment. Developmental Dynamics 240:413–421, 2011. © 2011 Wiley-Liss, Inc.

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