Patterns & Phenotypes
Wnt5a regulates growth, patterning, and odontoblast differentiation of developing mouse tooth
Article first published online: 18 JAN 2011
Copyright © 2011 Wiley-Liss, Inc.
Volume 240, Issue 2, pages 432–440, February 2011
How to Cite
Lin, M., Li, L., Liu, C., Liu, H., He, F., Yan, F., Zhang, Y. and Chen, Y. (2011), Wnt5a regulates growth, patterning, and odontoblast differentiation of developing mouse tooth. Dev. Dyn., 240: 432–440. doi: 10.1002/dvdy.22550
- Issue published online: 18 JAN 2011
- Article first published online: 18 JAN 2011
- Manuscript Accepted: 21 DEC 2010
- NIH. Grant Numbers: DE12329, DE15123
- National Natural Science Foundation of China. Grant Number: 30771132
- Ministry of Science and Technology of China. Grant Number: “973” Project 2010CB944800
- tooth development;
Wnt/β-catenin signaling is essential for tooth development beyond the bud stage, but little is known about the role of non-canonical Wnt signaling in odontogenesis. Here we compared the expression of Wnt5a, a representative of noncanonical Wnts, with that of Ror2, the Wnt5a receptor for non-canonical signaling, in the developing tooth, and analyzed tooth phenotype in Wnt5a mutants. Wnt5a-deficient mice exhibit retarded tooth development beginning from E16.5, leading to the formation of smaller and abnormally patterned teeth with a delayed odontoblast differentiation at birth. These defects are associated with upregulated Axin2 and Shh expression in the dental epithelium and reduced levels of cell proliferation in the dental epithelium and mesenchyme. Retarded tooth development and defective odontoblast differentiation were also observed in Ror2 mutant mice. Our results suggest that Wnt5a regulates growth, patterning, and odontoblast differentiation during odontogenesis, at least partially by modulating Wnt/β-catenin canonical signaling. Developmental Dynamics 240:432–440, 2011. © 2011 Wiley-Liss, Inc.