Special Issue Patterns & Phenotypes
Expression of key retinoic acid modulating genes suggests active regulation during development and regeneration of the amphibian limb
Article first published online: 2 FEB 2011
Copyright © 2011 Wiley-Liss, Inc.
Special Issue: Special Issue on Limb Development
Volume 240, Issue 5, pages 1259–1270, May 2011
How to Cite
McEwan, J., Lynch, J. and Beck, C. W. (2011), Expression of key retinoic acid modulating genes suggests active regulation during development and regeneration of the amphibian limb. Dev. Dyn., 240: 1259–1270. doi: 10.1002/dvdy.22555
- Issue published online: 20 APR 2011
- Article first published online: 2 FEB 2011
- Manuscript Accepted: 23 DEC 2010
- University of Otago Research Grant (2008)
Additional Supporting Information may be found in the online version of this article.
|DVDY_22555_sm_SuppFig1.tif||12400K||Supporting Information Figure 1. Whole mount in situ hybridisation showing expression of Crabp2 and Raldh2 in st. 56 hindlimbs and equivalent 3-day regenerates amputated at knee level at st. 56. Arrows indicate Crabp2 and Raldh2 expression in the anterior knee (A, C). Crabp2 (B) but not Raldh2 (D) shows expression in the pseudoblastema, which will not regenerate. White arrowheads mark the plane of amputation. Scale bars = 1 mm. Limbs are oriented with proximal to the left and posterior uppermost, and viewed from the dorsal side.|
|DVDY_22555_sm_SuppFig2.tif||5521K||Supporting Information Figure 2. Whole mount in situ hybridisation showing expression of Crabp2 and Raldh2 in 3-day tail regenerates for wildtype st. 50 (WT) transgenic st. 50 (N1) and refractory st. 47 (st 47) tadpoles. The animals were subjected to a heat shock at 34°C for 30 min and the posterior 50% of the tail was removed 3 hr later. Daily heat shocks were used to maintain transgene expression. Black arrowheads indicate Crabp2 and Raldh2 expression in the regenerating tail blastema (A, D). There is no regeneration and no expression of either gene in N1 tail stumps (B, E) or in refractory st. tails (C, F). White arrowheads, plane of amputation; yellow arrow in A, a pigment cell (melanophore). Scale bars = 1 mm. Tadpoles are oriented with anterior to the left and dorsal uppermost.|
|DVDY_22555_sm_SuppFig3.tif||7399K||Supporting Information Figure 3. Wholemount in situ hybridisation for Crabp2 and Raldh2 expression in wounded tadpole tails. Neither gene is expressed as a result of wounding. The yellow arrow indicates a melanophore and white arrows indicate the position of the wound site. Scale bars = 1 mm. Tadpoles are oriented with anterior to the left and dorsal uppermost.|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.