Additional Supporting Information may be found in the online version of this article.

DVDY_22556_sm_SuppFig1.tif8317KSupporting Information Figure 1. Pancreas development and impaired expansion of pancreatic tissue at earlier stages of post-PPx period in CLU−/−. Pancreas development was examined at 6 days (A, B) and 12 weeks (C, D) after birth both in WT (A, C) and CLU−/− mice (B, D). Pancreas of young and adult WT and CLU−/− mice showed a similar appearance in size and position (A–D) as well as in weight of adult pancreas (E, n=4). The pancreatic tissues (asterisks in C and D) surrounding splenic vessels indicated by arrows in F and H were removed for PPx from the mice shown in C and D, respectively. Pancreatic tissue surrounding splenic vessels (arrows in) was removed for PPx (F) from the same mice shown in and regeneration was traced 2 days after operation (G). From the CLU−/− mouse shown in D, approximately 85% of pancreatic tissue surrounding splenic vessel (arrows in D, H) was removed by PPx (H) and regeneration was traced 2 days after operation (I). Only a small pancreatic expansion (dotted area in I) was seen in the end of splenic vessel (arrow) in PPx-CLU−/−. In contrast, a prominent expansion of regenerating pancreas (dotted area in G) surrounding splenic vessel (arrow in G) was photographed at 2 days of post-PPx in WT mice (dotted area in G). The area was shown from the same mouse after operation. The histological sections of the pup (J, K) and adult pancreas (L, M) stained with insulin demonstrated normal development of islet beta-cells (arrows in J–M) with abundant exocrine tissues both in WT (J, L) and CLU−/− (K, M) mice.
DVDY_22556_sm_SuppFig2.tif4586KSupporting Information Figure 2. Integrated images of pancreatic tissues for morphometric analysis. The regenerating areas (asterisks in A and B) were discriminated by microscopic observation and marked on the photographs by dotted lines with lower magnification (×10). The percentage of regenerating area can be calculated by an image analyzing system. Regenerating areas of PPx-WT pancreas were larger than that of the PPx-CLU−/−. Scale bars = 200 μm.
DVDY_22556_sm_SuppFig3.tif8088KSupporting Information Figure 3. Determination of duct-rich and acinar-rich areas. The areas were determined by observation of double-stained section with CK-20 (green fluorescence) and amylase (red fluorescence). The areas were determined by immunoreactivity between CK-20 and amylase. A,B: Tissue areas showing predominant CCK-positive ducts are indicated by dotted lines (d), while the areas predominantly composed by amylase-positive cells are identified as an acinar-rich area (a). C: Higher magnification of A. Scale bars = 100 μm.

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