Sulfated is a negative feedback regulator of wingless in Drosophila
Article first published online: 8 FEB 2011
Copyright © 2011 Wiley-Liss, Inc.
Special Issue: Special Focus on Endoderm
Volume 240, Issue 3, pages 640–648, March 2011
How to Cite
You, J., Belenkaya, T. and Lin, X. (2011), Sulfated is a negative feedback regulator of wingless in Drosophila. Dev. Dyn., 240: 640–648. doi: 10.1002/dvdy.22562
- Issue published online: 17 FEB 2011
- Article first published online: 8 FEB 2011
- Manuscript Accepted: 28 DEC 2010
- 6-O endosulfatase;
Drosophila Wingless (Wg) acts as a morphogen to control pattern formation in a concentration dependent manner. Previous studies demonstrated important roles of heparan sulfate proteoglycans (HSPGs) in controlling Wg signaling and distribution. Here, we examined the role of Sulfated (Sulf1), a Drosophila homolog of vertebrate heparan sulfate 6-O endosulfatase, in Wg signaling and distribution. We show that sulf1 is specifically up-regulated by Wg signaling in the wing disc. We found that expression of Wg target gene senseless (sens) was elevated in the sulf1 mutant wing discs. Sulf1 also negatively regulate extracellular levels of Wg. Genetic interaction experiments indicate that Wg antagonist Notum may work synergistically with Sulf1 to restrict Wg signaling, and Dally, a member of Drosophila HSPGs, is a potential target of Sulf1. Our results demonstrate that sulf1 is a novel Wg target gene and by a feedback mechanism, it negatively regulated Wg signaling and distribution in vivo. Developmental Dynamics 240:640–648, 2011. © 2011 Wiley-Liss, Inc.