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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
DVDY_22567_sm_suppinfofig1.tif2128KSupp. Fig. S1. Alignment of mouse, human, and zebrafish Id1 promoter sequences corresponding to native bone morphogenic protein (BMP) response elements. Smad 1/5 binding sites, unknown protein binding sites, and Smad binding element sites are highlighted in red, blue and green, respectively. Asterisks indicate identical nucleotides between all three species. Numbers at the right side of the alignment indicate positions relative to transcriptional start sites based on Ensembl genome databases (mouse assembly version NCBI m37; human assembly version GRCh37; zebrafish assembly version Zv8).
DVDY_22567_sm_suppinfofig2.tif1046KSupp. Fig. S2. A–F: Treatment of 13- to 17-somite embryos with 50 μM dorsomorphin, or dimethyl sulfoxide (DMSO) control, for 3 hr down-regulates mRNA levels of bone morphogenic protein (BMP) -regulated genes by varying degrees: strongly, id1 (A,D); moderately, msxE (B,E); no observed change, hey1 (C,F).
DVDY_22567_sm_suppinfomov1.mov1924KSupp. Movie S1. Movie of Tg(BRE-AAVmlp:d2GFP)mw30; Tg(h2afx:h2afvmCherry)mw3 embryos from sphere stage to 18 somite stage. Confocal images were collected every 25 min to document expression of destabilized enhanced green fluorescent protein (d2GFP; green) and H2A-mCherry (red). Final movies show compressed Z-planes. The d2GFP is enriched in mesodermal tissues, particularly the lower trunk and tail.
DVDY_22567_sm_suppinfomov2.mov996KSupp. Movie S2. Movie of Tg(BRE-AAVmlp:d2GFP)mw30; Tg(h2afx:h2afvmCherry)mw3 embryos from 16 somite to 24 hours postfertilization (hpf). Confocal images were collected every 25 min to document expression of destabilized enhanced green fluorescent protein (d2GFP; green) and H2A-mCherry (red). Final movies shows compressed Z-planes. The d2GFP localizes to the heart and continues to express strongly in the tail. The d2GFP increases within the dorsal retina and lens.
DVDY_22567_sm_suppinfomov3.mov1172KSupp. Movie S3. Movie of Tg(BRE-AAVmlp:d2GFP)mw30; Tg(h2afx:h2afvmCherry)mw3 eye from 22 to 34 hours postfertilization (hpf). Confocal images were collected every 25 min to document expression of destabilized enhanced green fluorescent protein (d2GFP; green) and H2A-mCherry (red). Final movies shows compressed Z-planes. The d2GFP localizes to the dorsal part of the retina and lens during development. Note also d2GFP-positive cells in the trigeminal ganglion temporal to the eye, and the periocular mesenchyme moving anteriorly above the developing eye.

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