Tissue regeneration after injury in adult zebrafish: The regenerative potential of the caudal fin

Authors

  • Jinping Shao,

    1. Key Laboratory of Animal Models and Degenerative Neurological Diseases, Department of Physiology, Nankai University School of Medicine, Tianjin, China
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    • Jinping Shao and Dongyan Chen contributed equally to this article.

  • Dongyan Chen,

    1. Key Laboratory of Animal Models and Degenerative Neurological Diseases, Department of Physiology, Nankai University School of Medicine, Tianjin, China
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    • Jinping Shao and Dongyan Chen contributed equally to this article.

  • Qijun Ye,

    1. Key Laboratory of Animal Models and Degenerative Neurological Diseases, Department of Physiology, Nankai University School of Medicine, Tianjin, China
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  • Jianlin Cui,

    1. Key Laboratory of Animal Models and Degenerative Neurological Diseases, Department of Physiology, Nankai University School of Medicine, Tianjin, China
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  • Yuhao Li,

    1. Key Laboratory of Animal Models and Degenerative Neurological Diseases, Department of Physiology, Nankai University School of Medicine, Tianjin, China
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  • Lei Li

    Corresponding author
    1. Key Laboratory of Animal Models and Degenerative Neurological Diseases, Department of Physiology, Nankai University School of Medicine, Tianjin, China
    2. Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana
    • Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556
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Abstract

The zebrafish has the potential to regenerate many of its tissues. In this study, we examined caudal fin regeneration in zebrafish that received repeated injuries (fin amputation) at different ages. In zebrafish that received repeated injuries, the potential for caudal fin regeneration, such as tissue growth and the expression of regeneration marker genes (msxb, fgf20a, bmp2b), did not decline in comparison to zebrafish that received only one amputation surgery. The process of initial fin regeneration (e.g., tissue outgrowth and the expression of regeneration marker genes at 7 days post-amputation) did not seem to correlate with age. However, slight differences in fin outgrowth were observed between young and old animals when examined in the late regeneration stages (e.g., 20 and 30 days post-amputation). Together, the data suggest that zebrafish has unlimited regenerative potential in the injured caudal fin. Developmental Dynamics 240:1271–1277, 2011. © 2011 Wiley-Liss, Inc.

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