Patterns & Phenotypes
Evidence for partial epithelial-to-mesenchymal transition (pEMT) and recruitment of motile blastoderm edge cells during avian epiboly
Article first published online: 15 MAR 2011
Copyright © 2011 Wiley-Liss, Inc.
Volume 240, Issue 6, pages 1502–1511, June 2011
How to Cite
Futterman, M. A., García, A. J. and Zamir, E. A. (2011), Evidence for partial epithelial-to-mesenchymal transition (pEMT) and recruitment of motile blastoderm edge cells during avian epiboly. Dev. Dyn., 240: 1502–1511. doi: 10.1002/dvdy.22607
- Issue published online: 9 MAY 2011
- Article first published online: 15 MAR 2011
- Manuscript Accepted: 16 FEB 2011
- Georgia Tech Research Corporation
- epithelial migration;
- extracellular matrix;
- intermediate filaments;
Embryonic epiboly has become an important developmental model for studying the mechanisms underlying collective movements of epithelial cells. In the last couple of decades, most studies of epiboly have utilized Xenopus or zebrafish as genetically tractable model organisms, while the avian epiboly model has received virtually no attention. Here, we re-visit epiboly in quail embryos and characterize several molecular markers of epithelial-to-mesenchymal transition (EMT) in the inner zone of the extraembryonic Area Opaca and at the blastoderm edge. Our results show that the intermediate filament vimentin, a widely-used marker for the mesenchymal phenotype, is strongly expressed in the edge cells compared to the cells in the inner zone. Laminin, an extracellular matrix protein that is a major structural and adhesive component of the epiblast basement membrane and the inner zone of the Area Opaca, is notably absent from the blastoderm edge. While these expression profiles are consistent with a mesenchymal phenotype, several other epithelial markers, including cytokeratin, β-catenin, and E-cadherin, are present in the blastoderm edge cells. Moreover, the results of a BrDU proliferation assay strongly suggest that expansion of the edge cell population is primarily due to recruitment of cells from the inner zone, as opposed to proliferation. Taken together, our data show that the edge cells of the avian blastoderm have characteristics of both epithelial and mesenchymal cells, and that the avian epiboly model, which has been dormant for so many years, may yet again prove to be helpful as a unique developmental model for studying partial EMT in the context of collective epithelial cell migration. Developmental Dynamics 240:1502–1511, 2011. © 2011 Wiley-Liss, Inc.