Drs. Handschuh and Quintana contributed equally to this work.
Special Issue Research Article
Control of pelvic girdle development by genes of the Pbx family and Emx2
Article first published online: 31 MAR 2011
Copyright © 2011 Wiley-Liss, Inc.
Special Issue: Special Issue on Limb Development
Volume 240, Issue 5, pages 1173–1189, May 2011
How to Cite
Capellini, T. D., Handschuh, K., Quintana, L., Ferretti, E., Di Giacomo, G., Fantini, S., Vaccari, G., Clarke, S. L., Wenger, A. M., Bejerano, G., Sharpe, J., Zappavigna, V. and Selleri, L. (2011), Control of pelvic girdle development by genes of the Pbx family and Emx2. Dev. Dyn., 240: 1173–1189. doi: 10.1002/dvdy.22617
- Issue published online: 20 APR 2011
- Article first published online: 31 MAR 2011
- Manuscript Accepted: 22 FEB 2011
- NIH. Grant Numbers: 2RO1HD043997, 1RO1HD061403, R21DE018031-02S1, RO1HD059862
- March of Dimes and Birth Defects Foundation. Grant Number: 6-FY03-071
- Italian Association for Cancer Research
- ASM Foundation
- pelvis development;
Genes expressed in the somatopleuric mesoderm, the embryonic domain giving rise to the vertebrate pelvis, appear important for pelvic girdle formation. Among such genes, Pbx family members and Emx2 were found to genetically interact in hindlimb and pectoral girdle formation. Here, we generated compound mutant embryos carrying combinations of mutated alleles for Pbx1, Pbx2, and Pbx3, as well as Pbx1 and Emx2, to examine potential genetic interactions during pelvic development. Indeed, Pbx genes share overlapping functions and Pbx1 and Emx2 genetically interact in pelvic formation. We show that, in compound Pbx1;Pbx2 and Pbx1;Emx2 mutants, pelvic mesenchymal condensation is markedly perturbed, indicative of an upstream control by these homeoproteins. We establish that expression of Tbx15, Prrx1, and Pax1, among other genes involved in the specification and development of select pelvic structures, is altered in our compound mutants. Lastly, we identify potential Pbx1-Emx2–regulated enhancers for Tbx15, Prrx1, and Pax1, using bioinformatics analyses. Developmental Dynamics 240:1173–1189, 2011. © 2011 Wiley-Liss, Inc.