Special Issue Research Article
The chicken polydactyly (Po) locus causes allelic imbalance and ectopic expression of Shh during limb development
Article first published online: 4 APR 2011
Copyright © 2011 Wiley-Liss, Inc.
Special Issue: Special Issue on Limb Development
Volume 240, Issue 5, pages 1163–1172, May 2011
How to Cite
Dunn, I. C., Paton, I. R., Clelland, A. K., Sebastian, S., Johnson, E. J., McTeir, L., Windsor, D., Sherman, A., Sang, H., Burt, D. W., Tickle, C. and Davey, M. G. (2011), The chicken polydactyly (Po) locus causes allelic imbalance and ectopic expression of Shh during limb development. Dev. Dyn., 240: 1163–1172. doi: 10.1002/dvdy.22623
- Issue published online: 20 APR 2011
- Article first published online: 4 APR 2011
- Manuscript Accepted: 19 FEB 2011
- Biotechnology and Biological Sciences Research Council
- Sonic hedgehog;
Point mutations in the intronic ZRS region of Lmbr1, a limb specific cis-regulatory element of Sonic hedgehog (Shh), are associated with polydactyly in humans, cats, and mice. We and others have recently mapped the dominant preaxial polydactyly (Po) locus in Silkie chickens to a single nucleotide polymorphism (SNP) in the ZRS region. Using polymorphisms in the chicken Shh sequence, we confirm that the ZRS region directly regulates Shh expression in the developing limb causing ectopic Shh expression in the anterior leg, prolonged Shh expression in the posterior limb, and allelic imbalance between wt and Slk Shh alleles in heterozygote limbs. Using Silkie legs, we have explored the consequences of increased Shh expression in the posterior leg on the patterning of the toes, and the induction of preaxial polydactyly. Developmental Dynamics 240:1163–1172, 2011. © 2011 Wiley-Liss, Inc.