Regulative patterning in limb bud transplants is induced by distalizing activity of apical ectodermal ridge signals on host limb cells

Authors

  • Alberto Roselló-Díez,

    1. Departamento de Desarrollo y Reparación Cardiovascular, Centro Nacional de Investigaciones Cardiovasculares, Instituto de Salud Carlos III, Melchor Fernández Almagro, Madrid, Spain
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  • Miguel Torres

    Corresponding author
    1. Departamento de Desarrollo y Reparación Cardiovascular, Centro Nacional de Investigaciones Cardiovasculares, Instituto de Salud Carlos III, Melchor Fernández Almagro, Madrid, Spain
    • Departamento de Desarrollo y Reparación Cardiovascular, Centro Nacional de Investigaciones Cardiovasculares, Instituto de Salud Carlos III. Melchor Fernández Almagro, 3. E-28029 Madrid, Spain
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Abstract

We have used the chick limb as a model to gain insight into the longstanding question of regulative vs. mosaic development. To test the influence of signals on limb proximodistal development, distal limb bud tips of several stages were grafted to regions of the embryo known to provide different signaling environments. Of interest, thin grafts (100-micron thick) formed elements more proximal in character when grafted to the proximal limb region than when grafted to other regions. The extra elements were derived from host tissue, presumably distalized and recruited by the graft's apical ectodermal ridge signals. The results of classic and recent experiments have been reinterpreted in light of our conclusions. Developmental Dynamics 240:1203–1211, 2011. © 2011 Wiley-Liss, Inc.

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