Neural crest cells retain their capability for multipotential differentiation even after lineage-restricted stages
Article first published online: 18 MAY 2011
Copyright © 2011 Wiley-Liss, Inc.
Volume 240, Issue 7, pages 1681–1693, July 2011
How to Cite
Motohashi, T., Yamanaka, K., Chiba, K., Miyajima, K., Aoki, H., Hirobe, T. and Kunisada, T. (2011), Neural crest cells retain their capability for multipotential differentiation even after lineage-restricted stages. Dev. Dyn., 240: 1681–1693. doi: 10.1002/dvdy.22658
- Issue published online: 14 JUN 2011
- Article first published online: 18 MAY 2011
- Manuscript Accepted: 17 APR 2011
- Grants-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science. Grant Number: 20592086
- neural crest;
- embryonic stem cells;
Multipotency of neural crest cells (NC cells) is thought to be a transient phase at the early stage of their generation; after NC cells emerge from the neural tube, they are specified into the lineage-restricted precursors. We analyzed the differentiation of early-stage NC-like cells derived from Sox10-IRES-Venus ES cells, where the expression of Sox10 can be visualized with a fluorescent protein. Unexpectedly, both the Sox10+/Kit− cells and the Sox10+/Kit+ cells, which were restricted in vivo to the neuron (N)-glial cell (G) lineage and melanocyte (M) lineage, respectively, generated N, G, and M, showing that they retain multipotency. We generated mice from the Sox10-IRES-Venus ES cells and analyzed the differentiation of their NC cells. Both the Sox10+/Kit− cells and Sox10+/Kit+ cells isolated from these mice formed colonies containing N, G, and M, showing that they are also multipotent. These findings suggest that NC cells retain multipotency even after the initial lineage-restricted stages. Developmental Dynamics 240:1681–1693, 2011. © 2011 Wiley-Liss, Inc.