Role of paraxial mesoderm in limb/flank regionalization of the trunk lateral plate

Authors

  • Miyuki Noro,

    1. Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
    2. Laboratory for Vertebrate Body Plan, Center for Developmental Biology, RIKEN Kobe, Chuo-ku, Kobe, Japan
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  • Hiroki Yuguchi,

    1. Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
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  • Taeko Sato,

    1. Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
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  • Takanobu Tsuihiji,

    1. Department of Geology, National Museum of Nature and Science, Shinjuku-ku, Tokyo, Japan
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  • Sayuri Yonei-Tamura,

    1. Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
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  • Hitoshi Yokoyama,

    1. Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
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  • Yoshio Wakamatsu,

    1. Division of Developmental Neuroscience, Graduate School of Medicine, Tohoku University, Aoba, Sendai, Miyagi, Japan
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  • Koji Tamura

    Corresponding author
    1. Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
    • Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama Aoba-ku, Sendai 980-8578, Japan
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  • This article was accepted for inclusion in the Special Issue on Limb Development — Developmental Dynamics240#5.

Abstract

To understand the developmental mechanism that determines limb size and the consequent limb-to-trunk proportions in the tetrapod body, we investigated the role of the paraxial mesoderm in the specification of the limb and flank fields in the chick embryo. We found that the paraxial mesoderm subjacent to the limb field can affect the size of the limb bud along the anterior–posterior and proximal–distal axes. We also found that the paraxial mesoderm subjacent to the flank plays roles in suppressing the emergence and growth of the limb bud and in promoting flank-specific apoptosis in the lateral plate mesoderm. Our results suggest that signals from the paraxial mesoderm specify the limb and flank fields in the competent lateral plate mesoderm. Developmental Dynamics 240:1639–1649, 2011. © 2011 Wiley-Liss, Inc.

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