Gal80 intersectional regulation of cell-type specific expression in vertebrates
Article first published online: 8 SEP 2011
Copyright © 2011 Wiley-Liss, Inc.
Volume 240, Issue 10, pages 2324–2334, October 2011
How to Cite
Fujimoto, E., Gaynes, B., Brimley, C. J., Chien, C.-B. and Bonkowsky, J. L. (2011), Gal80 intersectional regulation of cell-type specific expression in vertebrates. Dev. Dyn., 240: 2324–2334. doi: 10.1002/dvdy.22734
- Issue published online: 19 SEP 2011
- Article first published online: 8 SEP 2011
- Manuscript Accepted: 19 AUG 2011
- NIH. Grant Numbers: R01 MH092256, K12 5HD001410, K08 DA024753, R01 EY12873
Additional Supporting Information may be found in the online version of this article.
|DVDY_22734_sm_SuppFigS1.tif||294K||Supporting Information Fig. S1. Schematic of positive and negative intersectional methods for labeling subgroups of cells. A: Positive intersectional method: a group of cells is labeled by partially overlapping expression domains of two different enhancers. Expression occurs only in cells in which both components (for example, of split Gal4) are present. B: Negative intersectional method: a group of cells is labeled by two different enhancers; expression occurs in the cells in which only the positive activator is present.|
|DVDY_22734_sm_SuppFigS2.tif||17570K||Supporting Information Fig. S2. Subgroups of neurons can be distinguished by inhibiting Gal4-driven expression with a fluorescently tagged Gal80. Single slice confocal images, lateral views, anterior to left, dorsal up, of 72 hours postfertilization (hpf) eyes in Tg(isl2b.3:Gal4)zc65; Tg(UAS:GFP) embryos. Immunostaining for green fluorescent protein (GFP), green; TagRFP, red; Topro3 nuclear stain, magenta. A–A″′: Embryos injected with hsp70l:TagRFP (no Gal80 expression). B–B″′: Embryos injected with hsp70l:Gal80-2A-TagRFP and heat-shocked at 48 hpf show expression of TagRFP (RFP) and inhibition of Gal4-dependent expression. C–C″′: Embryos injected with hsp70l:Gal80-TagRFP and heat-shocked at 48 hpf show RFP expression and concomitant inhibition of Gal4-dependent expression. (A″″–C″″) shows magnified view of the insets in (A″–C″), demonstrating disjoint GFP and RFP expression. Scale bar = 50 μm.|
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