Patterns & Phenotypes
Live imaging of endogenous periodic tryptophan protein 2 gene homologue during zebrafish development
Article first published online: 26 SEP 2011
Copyright © 2011 Wiley Periodicals, Inc.
Volume 240, Issue 11, pages 2578–2583, November 2011
How to Cite
Jayasena, C. S., Trinh, L. A. and Bronner, M. (2011), Live imaging of endogenous periodic tryptophan protein 2 gene homologue during zebrafish development. Dev. Dyn., 240: 2578–2583. doi: 10.1002/dvdy.22744
- Issue published online: 19 OCT 2011
- Article first published online: 26 SEP 2011
- Manuscript Accepted: 30 AUG 2011
- USPHS. Grant Number: P50HG004071
- ribosome biogenesis;
Yeast Periodic tryptophan protein 2 gene (Pwp2) is involved in ribosome biogenesis and has been implicated in regulation of the cell cycle in yeast. Here, we report a zebrafish protein-trap line that produces fluorescently tagged Periodic tryptophan protein 2 gene homologue (Pwp2h) protein, which can be dynamically tracked in living fish at subcellular resolution. We identified both full-length zebrafish Pwp2h and a short variant. The expression results show that Pwp2h is present in numerous sites in the early developing embryo, but later is restricted to highly proliferative regions, including the forebrain ventricular zone and endoderm-derived organs in the early larval stage. At the subcellular level, Pwp2h protein appears to be localized to the region of the nucleolus consistent with its presumed function in ribosomal RNA synthesis. This Pwp2h protein trap line offers a powerful tool to study the link between ribosome biogenesis and cell cycle progression during vertebrate development. Developmental Dynamics 240:2578–2583, 2011. © 2011 Wiley Periodicals, Inc.