Additional Supporting Information may be found in the online version of this article.

DVDY_22763_sm_SuppFig1.tif1494KSupp. Fig. S1. Pattern of GFP expression driven by the 24B-GAL4 driver. Detection of GFP expression by in situ hybridization in embryos carrying the 24B-GAL4 driver and the UAS-GFP transgene. A, B: Lateral (A) and dorsal (B) view of a germ band–extended embryo. C, D: Lateral (C) and dorsal (D) view of a germ band–retracted embryo. GFP is expressed in the whole mesoderm.
DVDY22763SuppFigS2.tif17974KSupp. Fig. S2. The eye phenotype caused by GATA4, Nkx2.5, TBX5, or pnr overexpression is 100% penetrant. Low-magnification images of flies overexpressing GATA4 (A), Nkx2.5 (B), TBX5 (C), or pnr (D) through the GMR-GAL4 driver at 18°C. All flies show narrow eyes.

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