Drs. Herrmann and Bundschu contributed equally to this work.
Tbx5 overexpression favors a first heart field lineage in murine embryonic stem cells and in Xenopus laevis embryos
Article first published online: 9 NOV 2011
Copyright © 2011 Wiley Periodicals, Inc.
Volume 240, Issue 12, pages 2634–2645, December 2011
How to Cite
Herrmann, F., Bundschu, K., Kühl, S. J. and Kühl, M. (2011), Tbx5 overexpression favors a first heart field lineage in murine embryonic stem cells and in Xenopus laevis embryos. Dev. Dyn., 240: 2634–2645. doi: 10.1002/dvdy.22776
- Issue published online: 9 NOV 2011
- Article first published online: 9 NOV 2011
- Accepted manuscript online: 17 OCT 2011 11:43AM EST
- Manuscript Accepted: 12 OCT 2011
- The International Graduate School in Molecular Medicine Ulm. Grant Number: GSC270
Additional Supporting Information may be found in the online version of this article.
|DVDY_22776_SuppFigS1.tif||411K||Supplementary Fig. 1. Vector map of the transfection vector for overexpressing Tbx5 in murine E14.1 ES cells. Tbx5 and EGFP are constitutively overexpressed. Their sequences are separated by an internal ribosomal entry site (IRES).|
|DVDY_22776_SuppFigS2.tif||1749K||Supplementary Fig. 2. Analysis of the sarcomere structure of cardiomyocytes differentiated until day 19 from wildtype and Tbx5 overexpressing ES cells. Cardiomyocytes were dispensed into single cells and subjected to immunofluorescent staining with either α-actinin (A) or cardiac Troponin T (B) antibody. Boxes mark the enlarged areas.|
|DVDY_22776_SuppMovieS1.mp4||2269K||Supplementary Movie 1. Beating phenotype of an embryoid body derived from wildtype E14.1 ES cells at day 14 of differentiation. Beating areas are locally restricted, beating frequency is low.|
|DVDY_22776_SuppMovieS2.mp4||532K||Supplementary Movie 2. Beating phenotype of an embryoid body overexpressing Tbx5 at day 14 of differentiation. Beating is spread over large areas of the embryoid body, beating is more frequent than observed in wildtype embryoid bodies.|
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