Additional Supporting Information may be found in the online version of this article.

DVDY_22776_SuppFigS1.tif411KSupplementary Fig. 1. Vector map of the transfection vector for overexpressing Tbx5 in murine E14.1 ES cells. Tbx5 and EGFP are constitutively overexpressed. Their sequences are separated by an internal ribosomal entry site (IRES).
DVDY_22776_SuppFigS2.tif1749KSupplementary Fig. 2. Analysis of the sarcomere structure of cardiomyocytes differentiated until day 19 from wildtype and Tbx5 overexpressing ES cells. Cardiomyocytes were dispensed into single cells and subjected to immunofluorescent staining with either α-actinin (A) or cardiac Troponin T (B) antibody. Boxes mark the enlarged areas.
DVDY_22776_SuppMovieS1.mp42269KSupplementary Movie 1. Beating phenotype of an embryoid body derived from wildtype E14.1 ES cells at day 14 of differentiation. Beating areas are locally restricted, beating frequency is low.
DVDY_22776_SuppMovieS2.mp4532KSupplementary Movie 2. Beating phenotype of an embryoid body overexpressing Tbx5 at day 14 of differentiation. Beating is spread over large areas of the embryoid body, beating is more frequent than observed in wildtype embryoid bodies.

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