Deficiency in crumbs homolog 2 (Crb2) affects gastrulation and results in embryonic lethality in mice

Authors

  • Zhijie Xiao,

    1. Department of Medical Biochemistry and Biophysics, Division of Matrix Biology, Karolinska Institutet, Stockholm, Sweden
    Search for more papers by this author
  • Jaakko Patrakka,

    1. Department of Medical Biochemistry and Biophysics, Division of Matrix Biology, Karolinska Institutet, Stockholm, Sweden
    Search for more papers by this author
  • Masatoshi Nukui,

    1. Department of Medical Biochemistry and Biophysics, Division of Matrix Biology, Karolinska Institutet, Stockholm, Sweden
    Search for more papers by this author
  • Lijun Chi,

    1. Department of Medical Biochemistry and Molecular Biology, Oulu Center for Cell Matrix Research, Biocenter Oulu, Laboratory of Developmental Biology, University of Oulu, Oulu, Finland
    Search for more papers by this author
  • Dadi Niu,

    1. Department of Medical Biochemistry and Biophysics, Division of Matrix Biology, Karolinska Institutet, Stockholm, Sweden
    Search for more papers by this author
  • Christer Betsholtz,

    1. Department of Medical Biochemistry and Biophysics, Division of Matrix Biology, Karolinska Institutet, Stockholm, Sweden
    Search for more papers by this author
  • Timo Pikkarainen,

    1. Department of Medical Biochemistry and Biophysics, Division of Matrix Biology, Karolinska Institutet, Stockholm, Sweden
    Search for more papers by this author
  • Seppo Vainio,

    Corresponding author
    1. Department of Medical Biochemistry and Molecular Biology, Oulu Center for Cell Matrix Research, Biocenter Oulu, Laboratory of Developmental Biology, University of Oulu, Oulu, Finland
    • Department of Biochemistry and Molecular Biology, University of Oulu, Oulu, Finland
    Search for more papers by this author
  • Karl Tryggvason

    Corresponding author
    1. Department of Medical Biochemistry and Biophysics, Division of Matrix Biology, Karolinska Institutet, Stockholm, Sweden
    • Department of Medical Biochemistry and Biophysics, Division of Matrix Biology, Karolinska Institutet, 171 77 Stockholm, Sweden
    Search for more papers by this author

Errata

This article is corrected by:

  1. Errata: Erratum: Deficiency in crumbs homolog 2 (Crb2) affects gastrulation and results in embryonic lethality in mice Volume 241, Issue 2, 431, Article first published online: 16 December 2011

Abstract

The Crumbs family of transmembrane proteins has an important role in the differentiation of the apical membrane domain in various cell types, regulating such processes as epithelial cell polarization. The mammalian Crumbs protein family is composed of three members. Here, we inactivated the mouse Crb2 gene with gene-targeting techniques and found that the protein is crucial for early embryonic development with severe abnormalities appearing in Crb2-deficient embryos at late-gastrulation. Our findings indicate that the primary defect in the mutant embryos is disturbed polarity of the epiblast cells at the primitive streak, which affects epithelial to mesenchymal transition (EMT) during gastrulation, resulting in impaired mesoderm and endoderm formation, and embryonic lethality by embryonic day 12.5. These findings therefore indicate a novel role for the Crumbs family of proteins. Developmental Dynamics 240:2646–2656, 2011. © 2011 Wiley Periodicals, Inc.

Ancillary