Additional Supporting Information may be found in the online version of this article.

DVDY_22781_sm_SuppFig1.tif12156KSupp. Fig. S1. Single-cell labeling of the spinal motor neuron in HGj4A larvae. A–C: The lateral view of the trunk region of the HGj4A larvae injected with the mnx1/hlxb9:mCherry plasmid. The arrowhead indicates the soma of a GFP-positive cell expressing RFP that locates slightly dorsal to the densely aligned GFP-positive cells in the ventral spinal cord. The dashed bracket indicates the motor axon innervating the skeletal muscles. In C, the GFP signal (A) and was merged into the RFP (B) signal. The inset in C shows the image of the same larvae taken with a shorter exposure time for RFP and indicates the single GFP-positive cell (arrowhead) is expressing RFP. Scale bars = 50 μm in A–C, 30 μm in the inset.
DVDY_22781_sm_SuppFig2.tif21070KSupp. Fig. S2. Transient expression of Gal4FF from the cis-regulatory element of the glyt2 gene in HGj4A;UAS:RFP larvae. A–C: The lateral view of the spinal cord of the HGj4A;UAS:RFP larvae injected with the glyt:GFF BAC construct at 48 hpf. The arrowheads indicate the somata of RFP-positive cells. In C, the RFP signal (A) and was merged into the GFP (B) signal. D: In total, 58 RFP-positive cells from five larvae were examined and, of these, two cells were GFP-positive. Scale bar = 30 μm in A–C.
DVDY_22781_sm_SuppInfo.doc27KSupporting Information

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