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DVDY_22782_sm_SuppFig1.tif8731KSupplementary Figure 1. A: Quantification of OTX expression shown in the western blot in Figure 1C. B: Western blot analysis of OTX factors expressed in S2 cell lines, indicating that they are expressed at similar levels. C: Western blot analysis of FLAG-tagged mutant CRX proteins from adult Drosophila head lysates. All mutant CRX proteins are expressed, albeit at different levels. Similarly to what was previously reported in vertebrate cell culture lines (Nichols et al., 2010), the CRX 138fs48 mutation increases the steady state levels of the protein. The R90W mutant has slightly reduced levels compared to wild-type CRX. Actin serves as a loading control.

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