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DVDY_23703_sm_SuppMovie1.MOV3698KSupp. Movie S1. Fibronectin matrix at DMM stage 1. The 3D reconstruction of one individual segment at DMM stage 1 isolated by manual contouring of confocal sections of a mouse embryo stained by whole mount immunofluorescence for myogenin/MHC (red) and fibronectin (green). The fibronectin matrix surrounds the dermomyotome and is present at intersegmental borders. A few bundles are seen in the interstitial space on the medial side of the myotome.
DVDY_23703_sm_SuppMovie2.MOV4445KSupp. Movie S2. Fibronectin matrix at DMM stage 2. The 3D reconstruction of one individual segment at DMM stage 2 isolated by manual contouring of confocal sections of a mouse embryo stained by whole mount immunofluorescence for myogenin/MHC (red) and fibronectin (green). The fibronectin matrix surrounds the dermomyotome and is present at intersegmental borders. A few bundles are seen in the interstitial space on the medial side of the myotome.
DVDY_23703_sm_SuppMovie3.MOV4520KSupp. Movie S3. Fibronectin matrix at DMM stage 3. The 3D reconstruction of one individual segment at DMM stage 3 isolated by manual contouring of confocal sections of a mouse embryo stained by whole mount immunofluorescence for MHC (red) and fibronectin (green). The fibronectin matrix is very dense around the dermomyotome, at intersegmental borders and on the medial side of the myotome.
DVDY_23703_sm_SuppMovie4.MOV4547KSupp. Movie S4. Fibronectin matrix at DMM stage 4. The 3D reconstruction of one individual segment at DMM stage 4 isolated by manual contouring of confocal sections of a mouse embryo stained by whole mount immunofluorescence for MHC (red) and fibronectin (green). The fibronectin matrix fills the interstitial space and is abundant within the muscle mass. It has invaded the developing dermis. The epaxial-most myocytes have started translocating.
DVDY_23703_sm_SuppMovie5.MOV4582KSupp. Movie S5. Tenascin matrix at DMM stage 2. The 3D reconstruction of one individual segment at DMM stage 2 isolated by manual contouring of confocal sections of a mouse embryo stained by whole mount immunofluorescence for myogenin/MHC (red) and tenascin (green). Note the restricted distribution pattern of the tenascin matrix; it is dense at the intersegmental border and forms bundles running along the myotome on its medial and lateral sides.
DVDY_23703_sm_SuppMovie6.MOV4525KSupp. Movie S6. Tenascin matrix at DMM stage 4. The 3D reconstruction of one individual segment at DMM stage 4 isolated by manual contouring of confocal sections of a mouse embryo stained by whole mount immunofluorescence for MHC (red) and tenascin (green). Myocytes insert in the abundant tenascin matrix at the intersegmental borders. Bundles of tenascin run along the myocytes on the lateral and medial sides of the myotome. The epaxial-most myocytes have started translocating.
DVDY_23703_sm_SuppMovie7.MOV5720KSupp. Movie S7. Tenascin matrix at E12.5. The 3D reconstruction of one individual segment of an E12.5 mouse embryo stained by whole-mount immunofluorescence MHC (red) and tenascin (green) showing the early epaxial muscle masses. Note the tenascin matrix at the sites of the tendon anlagen for the epaxial unisegmented transversospinalis muscles.
DVDY_23703_sm_SuppMovie8.MOV4580KSupp. Movie S8. Laminin matrix at DMM stage 1. The 3D reconstruction of one individual segment at DMM stage 1 isolated by manual contouring of confocal sections of a mouse embryo stained by whole mount immunofluorescence for myogenin/MHC (red) and laminin (green). The dermomyotome is digitally reconstructed and shown in grey. Note the two laminin matrices: the basement membrane of dermomyotome on the lateral side of the segment and the myotomal basement membrane on the medial side which is being assembled.
DVDY_23703_sm_SuppMovie9.MOV4546KSupp. Movie S9. Laminin matrix at DMM stage 3. The 3D reconstruction of one individual segment at DMM stage 3 isolated by manual contouring of confocal sections of a mouse embryo stained by whole mount immunofluorescence for myogenin/MHC (red) and laminin (green). Note the discontinuity of both dermomyotomal (lateral) and myotomal (medial) laminin matrices.
DVDY_23703_sm_SuppMovie10.MOV4560KSupp. Movie S10. Laminin matrix at DMM stage 4. The 3D reconstruction of one individual segment at DMM stage 4 isolated by manual contouring of confocal sections of a mouse embryo stained by whole-mount immunofluorescence for MHC (red) and laminin (green). Laminin matrices have been disassembled except in the most dorsal part which forms a pouch like structure. Myocytes are no longer contained by this pouch and several myocytes are no longer parallel to the axis. In fact, a few medial myocytes are dramatically off axis. Note the basement membranes of presumptive blood vessels around the segment.
DVDY_23703_sm_SuppMovie11.MOV5356KSupp. Movie S11. Laminin matrix at E12.5. The 3D reconstruction of one digitally isolated segment of an E12.5 mouse embryo stained by whole mount immunofluorescence for MHC (red) and laminin (green) showing the early epaxial muscle masses. The laminin matrices of the dermomyotome and the myotome are no longer detectable. Rather, the early epaxial muscle masses now contain spots of laminin immunoreactivity, most likely representing newly produced laminin which will later be assembled by the forming myotubes, giving rise to the basement membrane of the myofibers. Note the basement membranes of presumptive blood vessels around the muscle masses.

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