Mouse primitive streak forms in situ by initiation of epithelial to mesenchymal transition without migration of a cell population
Article first published online: 14 DEC 2011
Copyright © 2011 Wiley Periodicals, Inc.
Volume 241, Issue 2, pages 270–283, February 2012
How to Cite
Williams, M., Burdsal, C., Periasamy, A., Lewandoski, M. and Sutherland, A. (2012), Mouse primitive streak forms in situ by initiation of epithelial to mesenchymal transition without migration of a cell population. Dev. Dyn., 241: 270–283. doi: 10.1002/dvdy.23711
- Issue published online: 24 JAN 2012
- Article first published online: 14 DEC 2011
- Accepted manuscript online: 6 DEC 2011 02:26PM EST
- Manuscript Accepted: 20 NOV 2011
- NIH. Grant Numbers: RO1 HD034807, T32 GM008136
- NSF. Grant Number: IOS-1051294
Additional Supporting Information may be found in the online version of this article.
|DVDY_23711_sm_SuppFigS1.tif||1835K||Supporting Information Fig. S1. Germ layers can be identified by cell morphology in mT/mG embryos and embryo sections. A–C: Images of three different optical Z planes within an early-streak stage mT/mG embryo: A shows morphology of the epiblast layer, B shows morphology of the mesoderm layer, C shows morphology of the endoderm layer. Red is immuno-staining for T (Brachyury) to confirm mesoderm. Some cells within the nascent mesoderm layer demonstrate a round, bulbous appearance (arrowheads), which is the result of apical constriction as cells ingress at the streak.|
|DVDY_23711_sm_SuppMovieS1.mov||3375K||Supporting Information Movie S1. Time-lapse movie of a live e6.5 mT/mG embryo. This embryo is prestreak at the beginning of the movie, but is early streak (i.e., has mesoderm) by the movie's end.|
|DVDY_23711_sm_SuppMovieS2.mov||2641K||Supporting Information Movie S2. Time-lapse movie of a live no-bud stage mT/mG embryo. Primitive streak is seen en face at the level of the epiblast. Large, bulbous cells can be seen passing through the plane of view at varied times and locations as they ingress through the streak.|
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