Hyaluronan is required for cranial neural crest cells migration and craniofacial development
Article first published online: 19 DEC 2011
Copyright © 2011 Wiley Periodicals, Inc.
Volume 241, Issue 2, pages 294–302, February 2012
How to Cite
Casini, P., Nardi, I. and Ori, M. (2012), Hyaluronan is required for cranial neural crest cells migration and craniofacial development. Dev. Dyn., 241: 294–302. doi: 10.1002/dvdy.23715
- Issue published online: 24 JAN 2012
- Article first published online: 19 DEC 2011
- Accepted manuscript online: 6 DEC 2011 02:26PM EST
- Manuscript Accepted: 23 NOV 2011
Additional Supporting Information may be found in the online version of this article.
|DVDY_23715_sm_SuppFigS1.tif||9449K||Supp. Fig. S1. Molecular and morphological analysis of embryos injected with Has1, Has2, and CD44 control morpholinos. The injected side of each embryo is identified by the red-gal staining. A–F': Cranial NCC migration occurred normally in embryos injected with Has1-MoK, Has2-MoK, and CD44-MoK as revealed by the gene expression of Slug and Dll4. G, G', I, I', M, M': In embryos injected with Has1-MoK, Has2-MoK, and CD44-MoK TUNEL-positive cells are detected within the brain and the eye but never in the branchial arches region. H, L, N: The NCC-derived visceral skeleton developed correctly in Has1-MoK, Has2-MoK, and CD44-MoK embryos. e, eye; cg, cement gland.|
|DVDY_23715_sm_SuppFigS2.tif||12508K||Supp. Fig. S2. Control experiments: hyaluronan distribution in branchial arches of Has1 and Has2 stage-33 morphants. A–A''', B–B''': Cryostat horizontal sections at the branchial level of Has1 and Has2 morphants, showing the Hoechst staining to visualize nuclei (A, B), the GFP fluorescence to identify the injected side (A', B'), the biotynilated Hyaluronan Binding Complex (bHABC) staining to detect hyaluronan (A'', B''), and the merge (A''', B'''). In the injected side of morphants, a decreased amount of hyaluronan is present in the branchial extracellular spaces with respect to the uninjected counterpart. I-II-III-IV, branchial arches.|
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