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DVDY_23715_sm_SuppFigS1.tif9449KSupp. Fig. S1. Molecular and morphological analysis of embryos injected with Has1, Has2, and CD44 control morpholinos. The injected side of each embryo is identified by the red-gal staining. A–F': Cranial NCC migration occurred normally in embryos injected with Has1-MoK, Has2-MoK, and CD44-MoK as revealed by the gene expression of Slug and Dll4. G, G', I, I', M, M': In embryos injected with Has1-MoK, Has2-MoK, and CD44-MoK TUNEL-positive cells are detected within the brain and the eye but never in the branchial arches region. H, L, N: The NCC-derived visceral skeleton developed correctly in Has1-MoK, Has2-MoK, and CD44-MoK embryos. e, eye; cg, cement gland.
DVDY_23715_sm_SuppFigS2.tif12508KSupp. Fig. S2. Control experiments: hyaluronan distribution in branchial arches of Has1 and Has2 stage-33 morphants. A–A''', B–B''': Cryostat horizontal sections at the branchial level of Has1 and Has2 morphants, showing the Hoechst staining to visualize nuclei (A, B), the GFP fluorescence to identify the injected side (A', B'), the biotynilated Hyaluronan Binding Complex (bHABC) staining to detect hyaluronan (A'', B''), and the merge (A''', B'''). In the injected side of morphants, a decreased amount of hyaluronan is present in the branchial extracellular spaces with respect to the uninjected counterpart. I-II-III-IV, branchial arches.

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