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Additional Supporting Information may be found in the online version of this article.

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DVDY_23718_sm_SuppFigS1.tif796KSupp. Fig. S1. Distribution of branchiomotor neurons marked by Tg&|par;is|1:gfp)rw0 reporter expression at 3 dpf. Dorsa| whole mount with anterior to the left. A: Wild-type embryo. B: olig2 MO-injected embryo. Cranial motor neurons labeled “n.”
DVDY_23718_sm_SuppFigS2.tif9579KSupp. Fig. S2. Expression of isl RNA and protein in wild-type, control MO-injected, and olig2 MO-injected embryos. Dorsal whole mount with anterior to the left. A–C: isl1 RNA expression in 24-hpf embryos. D–F: Isl protein expression detected by immunocytochemistry in 24-hpf embryos. G–I: isl1 RNA expression in 33-hpf embryos. J–L: Isl protein expression detected by immunocytochemistry in 33-hpf embryos. M–O: isl1 RNA expression in 50-hpf embryos. P–R: Isl protein expression detected by immunocytochemistry in 50-hpf embryos. Otic vesicles are outlined.
DVDY_23718_sm_SuppFigS3.tif1784KSupp. Fig. S3. Tg(isl1:gfp)rw0 reporter expression reveals that the facial motor neuron migration defect in olig2 MO-injected embryos is not affected by co-injection of p53 MO. Panels are images showing dorsal views of whole embryos with anterior to the left. A–C: Wild-type embryos. D–F: olig2 MO-injected embryos. G–I: olig2 MO-injected embryos co-injected with p53 MO. Arrows indicate posteriorly migrated facial motor neurons. Bar indicates facial motor neurons along their caudal migration. Cranial motor neurons are labeled “n.”
DVDY_23718_sm_SuppFigS4.tif2480KSupp. Fig. S4. olig2 is not expressed in r4 and olig2 loss of function has no effect on hoxb1a or prickle1b expression. A: Wild-type expression at 22 hpf of olig2 in r5 and r6 in blue and egr2b in r3 and r5 in red. B: qPCR of hoxb1a expression in wild-type, control MO-injected, and olig2 MO-injected embryos at 24 and 33 hpf. C: qPCR of prickel1b expression in wild type, control MO-injected, and olig2 MO-injected embryos at 24 hpf. Error bars represent standard deviation. P value calculated by Student's t-test is listed above each bar and compared to wild-type embryos.
DVDY_23718_sm_SuppFigS5.tif782KSupp. Fig. S5. Glossopharyngeal motor neuron formation does not require olig2 function. Tg(olig2:EGFP) embryos labeled with anti-Alcama antibody (red) in wild-type and olig2 MO-injected embryos. Lateral sections, dorsal to the top, anterior to the left. A,B: Wild-type embryos. C,D: olig2 MO-injected embryos. Glossopharyngeal motor neurons indicated by arrows. Rhombomeres labeled “r.”

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