Drs. Liao and Wang contributed equally to this work.
Patterns & Phenotypes
Tol2 gene trap integrations in the zebrafish amyloid precursor protein genes appa and aplp2 reveal accumulation of secreted APP at the embryonic veins
Article first published online: 24 JAN 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 241, Issue 2, pages 415–425, February 2012
How to Cite
Liao, H.-K., Wang, Y., Noack Watt, K. E., Wen, Q., Breitbach, J., Kemmet, C. K., Clark, K. J., Ekker, S. C., Essner, J. J. and McGrail, M. (2012), Tol2 gene trap integrations in the zebrafish amyloid precursor protein genes appa and aplp2 reveal accumulation of secreted APP at the embryonic veins. Dev. Dyn., 241: 415–425. doi: 10.1002/dvdy.23725
- Issue published online: 24 JAN 2012
- Article first published online: 24 JAN 2012
- Accepted manuscript online: 19 DEC 2011 10:37AM EST
- Manuscript Accepted: 13 DEC 2011
- Center for Integrated Animal Genomics, ISU
- Roy J. Carver Charitable trust. Grant Number: 07-2991
- NIH-NCCR. Grant Number: P40 RR012546 to ZIRC
- NIH. Grant Number: DA14546
- Tol2 gene trap;
- endothelial cells;
- central nervous system
Background: The single spanning transmembrane amyloid precursor protein (APP) and its proteolytic product, amyloid-beta (Aβ) peptide, have been intensely studied due to their role in the pathogenesis of Alzheimer's disease. However, the biological role of the secreted ectodomain of APP, which is also generated by proteolytic cleavage, is less well understood. Here, we report Tol2 red fluorescent protein (RFP) transposon gene trap integrations in the zebrafish amyloid precursor protein a (appa) and amyloid precursor-like protein 2 (aplp2) genes. The transposon integrations are predicted to disrupt the appa and aplp2 genes to primarily produce secreted ectodomains of the corresponding proteins that are fused to RFP. Results: Our results indicate the Appa-RFP and Aplp2 fusion proteins are likely secreted from the central nervous system and accumulate in the embryonic veins independent of blood flow. Conclusions: The zebrafish appa and aplp2 transposon insertion alleles will be useful for investigating the biological role of the secreted form of APP. Developmental Dynamics 241:415–425, 2012. © 2012 Wiley Periodicals, Inc.