Inhibitory morphogens and monopodial branching of the embryonic chicken lung
Version of Record online: 23 MAR 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 241, Issue 5, pages 852–862, May 2012
How to Cite
Gleghorn, J. P., Kwak, J., Pavlovich, A. L. and Nelson, C. M. (2012), Inhibitory morphogens and monopodial branching of the embryonic chicken lung. Dev. Dyn., 241: 852–862. doi: 10.1002/dvdy.23771
- Issue online: 16 APR 2012
- Version of Record online: 23 MAR 2012
- Accepted manuscript online: 12 MAR 2012 01:03PM EST
- Manuscript Accepted: 27 FEB 2012
- David and Lucile Packard Foundation
- Alfred P. Sloan Foundation
- NIH. Grant Numbers: GM083997, HL110335
Additional Supporting Information may be found in the online version of this article.
|DVDY_23771_sm_SuppFig1.tif||6355K||Fig. S1. Endogenous transforming growth factor-beta1 (TGFβ1) and bone morphogenetic protein-4 (BMP4) signaling in the embryonic chicken lung. A,B: Shown are brightfield and fluorescence images for lungs stained for TGFβ1 (A) or BMP4 (B) and counterstained with DAPI (4′,6-diamidine-2-phenylidole-dihydrochloride). Representative images of immunofluorescence staining for (C) pSmad2/3 or pSmad1/5/8 and counterstained with DAPI in sections of lung explants (HH26–HH27).|
|DVDY_23771_sm_SuppFig2.tif||6470K||Fig. S2. Analysis of cell proliferation in treated explants. Shown are representative brightfield and fluorescent images of EdU incorporation in explanted lungs. The percentage of EdU-positive epithelial cells per field of view was quantified for at least 10 fields per explant and 3 explants per condition. Shown are mean ± SD. *P < 0.05, **P < 0.001 relative to control.|
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