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DVDY_23771_sm_SuppFig1.tif6355KFig. S1. Endogenous transforming growth factor-beta1 (TGFβ1) and bone morphogenetic protein-4 (BMP4) signaling in the embryonic chicken lung. A,B: Shown are brightfield and fluorescence images for lungs stained for TGFβ1 (A) or BMP4 (B) and counterstained with DAPI (4′,6-diamidine-2-phenylidole-dihydrochloride). Representative images of immunofluorescence staining for (C) pSmad2/3 or pSmad1/5/8 and counterstained with DAPI in sections of lung explants (HH26–HH27).
DVDY_23771_sm_SuppFig2.tif6470KFig. S2. Analysis of cell proliferation in treated explants. Shown are representative brightfield and fluorescent images of EdU incorporation in explanted lungs. The percentage of EdU-positive epithelial cells per field of view was quantified for at least 10 fields per explant and 3 explants per condition. Shown are mean ± SD. *P < 0.05, **P < 0.001 relative to control.

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