Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum

Authors

  • Nandor Nagy,

    1. Department of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
    2. Department of Human Morphology and Developmental Biology, Faculty of Medicine, Semmelweis University, Budapest, Hungary
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  • Alan J. Burns,

    1. Neural Development and Gastroenterology Units, UCL Institute of Child Health, London, United Kingdom
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  • Allan M. Goldstein

    Corresponding author
    1. Department of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
    • Massachusetts General Hospital, Warren 1153, Boston, MA 02114
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Abstract

A chick–chick intraspecies chimera was created by removing the neural tube adjacent to somites 2–6 from a normal chick embryo at E1.5 and replacing it with equivalent tissue from an age-matched chick-GFP transgenic embryo. At E10, the colorectum was removed, sectioned, and stained with HNK-1 antibody (red) to detect neural crest-derived cells, and with DAPI (blue) to label nuclei. Vagal neural crest-derived cells are HNK-1+/GFP+, while sacral neural crest derived-cells, which comprise the nerve of Remak, are HNK-1+/GFP−. From Nagy et al., Developmental Dynamics 241:842–851, 2012. © 2012 Wiley Periodicals, Inc.

Ancillary