Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum
Article first published online: 15 MAY 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 241, Issue 6, June 2012
How to Cite
Nagy, N., Burns, A. J. and Goldstein, A. M. (2012), Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum. Dev. Dyn., 241: n/a. doi: 10.1002/dvdy.23801
- Issue published online: 15 MAY 2012
- Article first published online: 15 MAY 2012
- NIH. Grant Number: R01DK080914
- Cited By
- enteric nervous system;
- Hirschsprung's disease;
- gut development;
A chick–chick intraspecies chimera was created by removing the neural tube adjacent to somites 2–6 from a normal chick embryo at E1.5 and replacing it with equivalent tissue from an age-matched chick-GFP transgenic embryo. At E10, the colorectum was removed, sectioned, and stained with HNK-1 antibody (red) to detect neural crest-derived cells, and with DAPI (blue) to label nuclei. Vagal neural crest-derived cells are HNK-1+/GFP+, while sacral neural crest derived-cells, which comprise the nerve of Remak, are HNK-1+/GFP−. From Nagy et al., Developmental Dynamics 241:842–851, 2012. © 2012 Wiley Periodicals, Inc.