John Herriges and Dr. Yi contributed equally to this work.
Patterns & Phenotypes
Genome-scale study of transcription factor expression in the branching mouse lung
Article first published online: 20 JUL 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 241, Issue 9, pages 1432–1453, September 2012
How to Cite
Herriges, J. C., Yi, L., Hines, E. A., Harvey, J. F., Xu, G., Gray, P. A., Ma, Q. and Sun, X. (2012), Genome-scale study of transcription factor expression in the branching mouse lung. Dev. Dyn., 241: 1432–1453. doi: 10.1002/dvdy.23823
- Issue published online: 22 AUG 2012
- Article first published online: 20 JUL 2012
- Accepted manuscript online: 18 JUN 2012 12:14PM EST
- Manuscript Accepted: 5 JUN 2012
- Burroughs-Wellcome. Grant Number: 1002361
- American Heart Association. Grant Number: 0950041G
- March of Dimes. Grant Number: 6-FY10-339
- NHLBI. Grant Number: HL113870
- NSFC. Grant Numbers: 31028012, 11PRE5540006, 0610087Z
- National Science Foundation. Grant Number: 2011101268
- transcription factors;
- expression patterns;
Background: Mammalian lung development consists of a series of precisely choreographed events that drive the progression from simple lung buds to the elaborately branched organ that fulfills the vital function of gas exchange. Strict transcriptional control is essential for lung development. Among the large number of transcription factors encoded in the mouse genome, only a small portion of them are known to be expressed and function in the developing lung. Thus a systematic investigation of transcription factors expressed in the lung is warranted. Results: To enrich for genes that may be responsible for regional growth and patterning, we performed a screen using RNA in situ hybridization to identify genes that show restricted expression patterns in the embryonic lung. We focused on the pseudoglandular stage during which the lung underergoes branching morphogenesis, a cardinal event of lung development. Using a genome-scale probe set that represents over 90% of the transcription factors encoded in the mouse genome, we identified 62 transcription factor genes with localized expression in the epithelium, mesenchyme, or both. Many of these genes have not been previously implicated in lung development. Conclusions: Our findings provide new starting points for the elucidation of the transcriptional circuitry that controls lung development. Developmental Dynamics 241:1432–1453, 2012. © 2012 Wiley Periodicals, Inc.