Ryohei Nagai and Mai Shinomura contributed equally to this work.
Patterns & Phenotypes
Dynamics of GFRα1-positive spermatogonia at the early stages of colonization in the recipient testes of W/Wν male mice
Version of Record online: 9 JUL 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 241, Issue 8, pages 1374–1384, August 2012
How to Cite
Nagai, R., Shinomura, M., Kishi, K., Aiyama, Y., Harikae, K., Sato, T., Kanai-Azuma, M., Kurohmaru, M., Tsunekawa, N. and Kanai, Y. (2012), Dynamics of GFRα1-positive spermatogonia at the early stages of colonization in the recipient testes of W/Wν male mice. Dev. Dyn., 241: 1374–1384. doi: 10.1002/dvdy.23824
- Issue online: 17 JUL 2012
- Version of Record online: 9 JUL 2012
- Accepted manuscript online: 28 JUN 2012 01:06PM EST
- Manuscript Accepted: 11 JUN 2012
- Grants-in-Aid for Scientific Research (KAKENHI). Grant Numbers: 30260326, 23132504, 21116501, 50431838, 10J01100
- spermatogonial stem cells;
- spermatogonial transplantation;
Background: The spermatogonial transplantation experiment can be used as an unequivocal detection assay of spermatogenic stem cells (SSCs) in both a qualitative and quantitative manner, based on their regenerative capacity. In this study, the proliferative patterns and kinetics of donor-derived GFRα1-positive spermatogonia containing potential SSCs were examined during early colonization following spermatogonial transplantation.Results: Donor-derived GFRα1-positive cells frequently formed several aggregates of Aal(aligned)/morula-like structures in a single spermatogenic cell patch before and on day 14 post-transplant, indicating a possible involvement in the formation of a stable spermatogenic colony at 21 days post-transplant. The appearance of these Aal/morula-like aggregates is positively correlated with regional, high-level expression of immunoreactive GDNF signals, a ligand for GFRα1, associated with colony expansion. Conclusions: These data raise the hypothesis that regional GDNF signals regulate the balance between donor-derived Aal-like cell aggregates and their differentiation in each small patch, which subsequently leads to further selection of survival colonies at later stages. Developmental Dynamics 241:1374–1384, 2012. © 2012 Wiley Periodicals, Inc.