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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
DVDY_23824_sm_SuppFig1.tif1749KSupporting Information Figure 1. GFP+ spermatogenic patches without any GFRα1-positive cells in the transplanted W/Wv seminiferous tubules on days 7 to 14 post-transplant. Whole-mount anti-GFRα1 immunostaining (red fluorescence) of tubule fragments containing GFP+ clusters (green fluorescence) from transplanted W/Wv mouse testes (broken lines demarcate the outline of the seminiferous tubules). Red and green numbers (in right lower corner of each panel) represent the number of GFRα1-positive cells and total number of GFP+ cells in each GFP+ patch, respectively.
DVDY_23824_sm_SuppFig2.tif846KSupporting Information Figure 2. Three focus plane images of a single GFP+ patch in transplanted W/Wv seminiferous tubules on day 14 post-transplant. Whole-mount anti-GFRα1 immunostaining (red fluorescence; arrowheads in right panels in B) of the tubule fragment containing a single GFP+ cluster (green fluorescence; left panels in B) from transplanted W/Wv mouse testes at three distinct z-stack planes (from surface to bottom, as shown in A). Asterisks indicate non-specific signals in the interstitial regions. Scale bars = 100 μm.

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