Ryohei Nagai and Mai Shinomura contributed equally to this work.
Patterns & Phenotypes
Dynamics of GFRα1-positive spermatogonia at the early stages of colonization in the recipient testes of W/Wν male mice
Article first published online: 9 JUL 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 241, Issue 8, pages 1374–1384, August 2012
How to Cite
Nagai, R., Shinomura, M., Kishi, K., Aiyama, Y., Harikae, K., Sato, T., Kanai-Azuma, M., Kurohmaru, M., Tsunekawa, N. and Kanai, Y. (2012), Dynamics of GFRα1-positive spermatogonia at the early stages of colonization in the recipient testes of W/Wν male mice. Dev. Dyn., 241: 1374–1384. doi: 10.1002/dvdy.23824
- Issue published online: 17 JUL 2012
- Article first published online: 9 JUL 2012
- Accepted manuscript online: 28 JUN 2012 01:06PM EST
- Manuscript Accepted: 11 JUN 2012
- Grants-in-Aid for Scientific Research (KAKENHI). Grant Numbers: 30260326, 23132504, 21116501, 50431838, 10J01100
Additional Supporting Information may be found in the online version of this article.
|DVDY_23824_sm_SuppFig1.tif||1749K||Supporting Information Figure 1. GFP+ spermatogenic patches without any GFRα1-positive cells in the transplanted W/Wv seminiferous tubules on days 7 to 14 post-transplant. Whole-mount anti-GFRα1 immunostaining (red fluorescence) of tubule fragments containing GFP+ clusters (green fluorescence) from transplanted W/Wv mouse testes (broken lines demarcate the outline of the seminiferous tubules). Red and green numbers (in right lower corner of each panel) represent the number of GFRα1-positive cells and total number of GFP+ cells in each GFP+ patch, respectively.|
|DVDY_23824_sm_SuppFig2.tif||846K||Supporting Information Figure 2. Three focus plane images of a single GFP+ patch in transplanted W/Wv seminiferous tubules on day 14 post-transplant. Whole-mount anti-GFRα1 immunostaining (red fluorescence; arrowheads in right panels in B) of the tubule fragment containing a single GFP+ cluster (green fluorescence; left panels in B) from transplanted W/Wv mouse testes at three distinct z-stack planes (from surface to bottom, as shown in A). Asterisks indicate non-specific signals in the interstitial regions. Scale bars = 100 μm.|
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