Patterns & Phenotypes
ETV2 expression marks blood and endothelium precursors, including hemogenic endothelium, at the onset of blood development
Article first published online: 16 JUL 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 241, Issue 9, pages 1454–1464, September 2012
How to Cite
Wareing, S., Eliades, A., Lacaud, G. and Kouskoff, V. (2012), ETV2 expression marks blood and endothelium precursors, including hemogenic endothelium, at the onset of blood development. Dev. Dyn., 241: 1454–1464. doi: 10.1002/dvdy.23825
- Issue published online: 22 AUG 2012
- Article first published online: 16 JUL 2012
- Accepted manuscript online: 25 JUN 2012 11:06AM EST
- Manuscript Accepted: 12 JUN 2012
- Cancer Research UK core-funded grant and PhD fellowship
- Biotechnology and Biological Sciences Research Council grant
- hemogenic endothelium;
- yolk sac hematopoiesis;
Background: ETV2 has been identified as an important player in embryonic hematopoiesis. However, the cell populations in which this transcription factor is expressed and operates during blood specification remain to be fully characterized. Here we address these issues using ES cells and a transgenic mouse line expressing green fluorescent protein (GFP) under the control of ETV2 regulatory elements, allowing us to observe the tight association between ETV2 expression and the initiation of hematopoiesis. Results: Both in differentiating ES cells and gastrulating embryos ETV2::GFP is mostly found co-expressed with endothelial markers and defines a subset of cells with greatly enriched primitive erythroid potential. Upon culture ETV2::GFP cells rapidly up-regulate CD41, down-regulate endothelium cell surface markers and generate definitive hematopoietic progenitors. Altogether these characteristics represent the hallmark of hemogenic endothelium cells, a specialized endothelium originating from the hemangioblast and giving rise to hematopoietic cells. Importantly, ETV2 deficiency results in a complete absence of hemogenic endothelium in differentiating ES cells and gastrulating embryos. Conclusions: Altogether our data reveal that ETV2 marks hemogenic endothelium in gastrulating embryos and is absolutely required for the formation of this precursor at the onset of hematopoiesis. These results enhance our understanding of embryonic hematopoiesis and the factors driving hemogenic endothelium specification. Developmental Dynamics 241:1454–1464, 2012. © 2012 Wiley Periodicals, Inc.