Rene Rezsöhazy and Isabelle Donnay contributed equally to this study.
Patterns & Phenotypes
Characterization of TALE genes expression during the first lineage segregation in mammalian embryos
Article first published online: 10 OCT 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 241, Issue 11, pages 1827–1839, November 2012
How to Cite
Sonnet, W., Rezsöhazy, R. and Donnay, I. (2012), Characterization of TALE genes expression during the first lineage segregation in mammalian embryos. Dev. Dyn., 241: 1827–1839. doi: 10.1002/dvdy.23873
- Issue published online: 17 OCT 2012
- Article first published online: 10 OCT 2012
- Accepted manuscript online: 17 SEP 2012 02:37PM EST
- Manuscript Accepted: 8 SEP 2012
- FRFC grant from the Fonds National de la Recherche Scientifique. Grant Number: 2456607
- homeodomain proteins;
- preimplantation development;
Background: Three amino acid loop extension (TALE) homeodomain-containing transcription factors are generally recognized for their role in organogenesis and differentiation during embryogenesis. However, very little is known about the expression and function of Meis, Pbx, and Prep genes during early development. Results: In order to determine whether TALE proteins could contribute to the early cell fate decisions in mammalian development, this study aimed to characterize in a systematic manner the pattern of expression of all Meis, Pbx, and Prep genes from the precompaction to blastocyst stage corresponding to the first step of cell differentiation in mammals. To reveal to what extent TALE genes expression at these early stages is a conserved feature among mammals, this study was performed in parallel in the bovine and mouse models. We demonstrated the transcription and translation of TALE genes, before gastrulation in the two species. At least one member of Meis, Pbx, and Prep subfamilies was found expressed at the RNA and protein levels but different patterns of expression were observed between genes and between species, suggesting specific gene regulations. Conclusions: Taken together, these results suggest a previously unexpected involvement of these factors during the early development in mammals. Developmental Dynamics 241:1827–1835, 2012. © 2012 Wiley Periodicals, Inc.