Ephrin-B stimulation of calvarial bone formation
Article first published online: 5 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Special Issue: Special Focus on Developmental Biology in Latin America
Volume 241, Issue 12, pages 1901–1910, December 2012
How to Cite
Benson, M. D., Opperman, L. A., Westerlund, J., Fernandez, C. R., San Miguel, S., Henkemeyer, M. and Chenaux, G. (2012), Ephrin-B stimulation of calvarial bone formation. Dev. Dyn., 241: 1901–1910. doi: 10.1002/dvdy.23874
- Issue published online: 20 NOV 2012
- Article first published online: 5 NOV 2012
- Manuscript Accepted: 28 AUG 2012
- NIDCR. Grant Number: R03DE020119
Additional Supporting Information may be found in the online version of this article.
|DVDY_23874_sm_SuppFig1.tif||8219K||Supporting Information Figure 1. Blocking of ephrin-A5 induced growth cone turning by unclustered EphA4/Fc recombinant protein. Ephrin-A5/Fc protein or IgG Fc protein clustered with Cy3-conjugated anti-Fc was laid down in stripes on poly-ornithine-coated glass by the Bonhoeffer method (Nature Protocols 2:1216). Hippocampal neurons were isolated from perinatal mice (Nature Protocols 2:1490) were plated on top of the stripes in the presence of soluble EphA4/Fc or IgG Fc (as control) and allowed to grow for 48 hr. Cells were fixed and stained with an antibody to acetylated tubulin to visualize neurites. A: Ten images as shown here were taken of each group. A blinded observer scored each axon in each panel for turning or stopping when it encountered a red stripe, and determined a % turning score for each group. B: Frequency of neurite turning represented as fold difference relative to that observed on control IgG Fc stripes for ephrin-A5/Fc stripes and ephrin-A5/Fc stripes incubated with unclustered EphA4/Fc protein. The EphA4 protein effectively blocked the neurite turning activity of ephrin-A5 that would normally stop or turn away axonal growth from hippocampal neurons (J Neurosci 20:8885).|
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