Functional redundancy between Cdc14 phosphatases in zebrafish ciliogenesis
Version of Record online: 25 OCT 2012
Copyright © 2012 Wiley Periodicals, Inc.
Special Issue: Special Focus on Developmental Biology in Latin America
Volume 241, Issue 12, pages 1911–1921, December 2012
How to Cite
Clément, A., Solnica-Krezel, L. and Gould, K. L. (2012), Functional redundancy between Cdc14 phosphatases in zebrafish ciliogenesis. Dev. Dyn., 241: 1911–1921. doi: 10.1002/dvdy.23876
- Issue online: 20 NOV 2012
- Version of Record online: 25 OCT 2012
- Accepted manuscript online: 1 OCT 2012 11:51AM EST
- Manuscript Accepted: 20 SEP 2012
Additional Supporting Information may be found in the online version of this article.
|DVDY_23876_sm_SuppFig1.tif||1458K||Supporting Information Figure 1. Expression of cdc14a1. A: Sequences targeted by cdc14a1 transcript specific probes (purple bars). B–F: In situ hybridization of cdc14a1_tv1. G–K: In situ hybridization of cdc14a1_tv2. Scale bar =500 μm. L: Temporal expression of cdc14a1_tv1 and cdc14a1_tv2 by RT-PCR.|
|DVDY_23876_sm_SuppFig2.tif||1584K||Supporting Information Figure 2. Control of injection procedure. A,B: Comparison of morphological phenotypes between non-injected (NI) (A) and H2O-injected (B) embryos at 31 hpf. C: Quantification of body trunk curvature at 1 dpf and 3 dpf. D,E: Rescue of body curvature at 31 hpf. Body shape phenotype (D) and quantification (E). Scale bar = 100 μm. The numbers of embryos analyzed are indicated. Data are represented as mean ± SEM. Statistics were done using Student's t-Test.|
|DVDY_23876_sm_SuppFig3.tif||261K||Supporting Information Figure 3. Effect of Cdc14A1 loss of function on kidney cilia and inner ear kinocilia. A: Cilia length in the posterior kidney duct was determined at 31 hpf. B: Cilia length in the posterior macula of inner ear at 5 dpf. The numbers of cilia analyzed are indicated. Data are represented as mean ± SEM. Statistics were done using Student's t-Test.|
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