Patterns & Phenotypes
During Embryogenesis, Esrp1 Expression Is Restricted to a Subset of Epithelial Cells and Is Associated With Splicing of a Number of Developmentally Important Genes
Article first published online: 30 JAN 2013
Copyright © 2012 Wiley Periodicals, Inc.
Volume 242, Issue 3, pages 281–290, March 2013
How to Cite
Revil, T. and Jerome-Majewska, L. A. (2013), During Embryogenesis, Esrp1 Expression Is Restricted to a Subset of Epithelial Cells and Is Associated With Splicing of a Number of Developmentally Important Genes. Dev. Dyn., 242: 281–290. doi: 10.1002/dvdy.23918
- Issue published online: 20 FEB 2013
- Article first published online: 30 JAN 2013
- Accepted manuscript online: 10 DEC 2012 08:32AM EST
- Manuscript Accepted: 28 NOV 2012
- Canadian Institutes of Health Research . Grant Number: MOP-102666
- Centre for the Study of Reproduction
- alternative splicing;
- pharyngeal pouches
Background: Development of a mature organism from a single cell requires a series of important morphological changes, which is in part regulated by alternative splicing. In this article, we report the expression of Esrp1 during early mouse embryogenesis, a splicing factor implicated in epithelial to mesenchymal transitions. Results: By qRT-PCR, we find higher expression of Esrp1 and Esrp2 in placenta compared to the embryos. We also find a correlation between the expression of Esrp1 and alternative splicing of several known target exons. Using in situ RNA hybridization we show that while Esrp1 expression is ubiquitous in embryonic day (E)6.5 mouse embryos, expression becomes restricted to the chorion and definitive endoderm starting at E7.5. Esrp1 expression was consistently restricted to a subset of epithelial cell types in developing embryos from E9.5 to E13.5. Conclusions: Our results suggest that Esrp1 could play an important role in the morphological changes underlying embryogenesis of the placenta and embryo. Developmental Dynamics 242:281–290, 2013. © 2012 Wiley Periodicals, Inc.