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FilenameFormatSizeDescription
dvdy23925-sup-0001-suppinfo.tif796KFig. S1. At 7 days exposed to a new environment, both corneal epithelium and early dorsal epidermis differentiated according to their already determined fate. A: After 2 weeks, the corneal epithelium (one cell layer) recombined with a dorsal dermis (de) stratified but showed its characteristic corneal features. B,C: Compare with the reverse case (B): the epidermis, which was also one cell layer at the time of its recombination with a corneal stroma (st) showed characteristic stratified layers, from the basal layer (bl) to the stratified cornified layers (stc), and did not expressed Pax6 (C).
dvdy23925-sup-0002-suppinfo.tif1512KFig. S2. A–G: Surgical ablation of the chick lens pit at Hamburger and Hamilton (HH) stage 14 can also (compare with Fig. 3) lead to an almost normal eye (A), or an eye with an abnormal lens (B–G). A: In 35% of cases, the right operated eye (black arrow) was simply smaller than the control left eye (white arrows). B–G: In 35% of cases, the eye was abnormal (B), the renewed lens (le) was very small or, as in the case illustrated, remained open (D). However, its associated ectoderm differentiated into a corneal epithelium (ce), recognized by its characteristic histological features: all the cells are similar throughout its thickness (E,G) and express K12 (C). In contrast, the epidermis (epd) of the eyelids (eyl) shows a basal cell layer (bl) (F). ir: iris; cj, conjunctive
dvdy23925-sup-0003-suppinfo.tif1089KFig. S3. When the lens preplacode is ablated at Hamburger and Hamilton (HH) stage 13 (18 somites) in the right eye, the morphogenesis of the renewed lens is delayed when compared with the control left eye. A: Five hours after surgical ablation, the control left eye formed a thick lens placode (lp), while on the right side the wound healing ectoderm is still very thin. B,C: Six hours after the surgical ablation, on the left a lens vesicle (lv) had already formed (B), while on the right eye (C) there is still no lens placode formation. Note that, in both eyes, the optic cup has formed.
dvdy23925-sup-0004-suppinfo.tif852KFig. S4. Overexpression of Gremlin at Hamburger and Hamilton (HH) stage 16 after RCAS-Gremlin electroporation at HH stage 10. A: At HH stage 10, just after electroporation, fast green staining of the eye ectoderm, in front of the optic vesicle (ov). B: At HH stage 16, 14h after the electroporation, the right head area all strongly expresses Gremlin, involving the eye area (white dashes). C: In a control embryo, note that Gremlin expression in the head mesenchyme occurs mainly in a circle around the eye (ey). np: nasal pit.

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