Dr. Horiguchi's current address is Department of Biology, Faculty of Medicine, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431-3192, Japan.
Special Issue Research Article
Characterization of gonadal soma-derived factor expression during sex change in the protogynous wrasse, Halichoeres trimaculatus
Article first published online: 1 MAR 2013
Copyright © 2013 Wiley Periodicals, Inc.
Special Issue: Sex Determination
Volume 242, Issue 4, pages 388–399, April 2013
How to Cite
Horiguchi, R., Nozu, R., Hirai, T., Kobayashi, Y., Nagahama, Y. and Nakamura, M. (2013), Characterization of gonadal soma-derived factor expression during sex change in the protogynous wrasse, Halichoeres trimaculatus. Dev. Dyn., 242: 388–399. doi: 10.1002/dvdy.23929
Dr. Nakamura's current address is Okinawa Churashima Foundation, 888 Ishikawa, Motobu, Okinawa 905-0206, Japan.
- Issue published online: 20 MAR 2013
- Article first published online: 1 MAR 2013
- Accepted manuscript online: 17 JAN 2013 10:43PM EST
- Manuscript Revised: 4 JAN 2013
- Manuscript Accepted: 4 JAN 2013
- Manuscript Received: 2 JUL 2012
- Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science. Grant Numbers: 24770058, 23248034
- SORST Research Project of Japan Science and Technology Corporation
- the 21st Century COE project of the University of the Ryukyus, Japan
- sex change;
- sexual plasticity;
- three-spot wrasse
Background: Sex change in fishes provides a good experimental model for understanding the mechanisms and plasticity of sex determination and differentiation. The three-spot wrasse, Halichoeres trimaculatus is a protogynous hermaphrodite. During sex change from female to male, the ovary is replaced by the testis through the degeneration of oocytes and subsequent spermatogenesis. In the present study, we cloned a cDNA-encoding gonadal soma-derived factor (GSDF) from protogynous wrasse and examined its expression pattern in the sexually mature gonads and the sex-changing gonad induced experimentally by aromatase inhibition. Results: Expression of gsdf was predominantly observed in the testis, and it was mainly localized to the supporting cells surrounding the spermatogonia. In the ovary, only slight expression of gsdf was observed in morphologically undifferentiated supporting cells in contact with oogonia. During sex change, strong expression of gsdf appeared first in the supporting cells surrounding the gonial germ cells before the onset of spermatogenesis. Thereafter, the expression of gsdf continually increased in the supporting cells surrounding the proliferating spermatogonia throughout the sex change. Conclusions: These results suggest that gsdf is involved in the proliferation of spermatogonia and subsequent spermatogenesis in both the testis and the gonad in the early stages of sex change. Developmental Dynamics 242:388–399, 2013. © 2013 Wiley Periodicals, Inc.