Characterization of gonadal soma-derived factor expression during sex change in the protogynous wrasse, Halichoeres trimaculatus

Authors

  • Ryo Horiguchi,

    Corresponding author
    1. Solution-Oriented Research for Science and Technology, Japan Science and Technology Corporation, Saitama, Japan
    2. Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, Motobu, Okinawa, Japan
    • Laboratory of Reproductive Biology, National Institution for Basic Biology, Okazaki, Aichi, Japan
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  • Ryo Nozu,

    1. Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, Motobu, Okinawa, Japan
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  • Toshiaki Hirai,

    1. Department of Bioscience/Biotechnology Research Center, Teikyo University of Science and Technology, Uenohara, Yamanashi, Japan
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  • Yasuhisa Kobayashi,

    1. Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, Motobu, Okinawa, Japan
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  • Yoshitaka Nagahama,

    1. Laboratory of Reproductive Biology, National Institution for Basic Biology, Okazaki, Aichi, Japan
    2. Solution-Oriented Research for Science and Technology, Japan Science and Technology Corporation, Saitama, Japan
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  • Masaru Nakamura

    1. Solution-Oriented Research for Science and Technology, Japan Science and Technology Corporation, Saitama, Japan
    2. Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, Motobu, Okinawa, Japan
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  • Dr. Horiguchi's current address is Department of Biology, Faculty of Medicine, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431-3192, Japan.

  • Dr. Nakamura's current address is Okinawa Churashima Foundation, 888 Ishikawa, Motobu, Okinawa 905-0206, Japan.

Correspondence to: Ryo Horiguchi, Department of Biology, Faculty of Medicine, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431-3192, Japan. E-mail, horigu@xb3.so-net.ne.jp

Abstract

Background: Sex change in fishes provides a good experimental model for understanding the mechanisms and plasticity of sex determination and differentiation. The three-spot wrasse, Halichoeres trimaculatus is a protogynous hermaphrodite. During sex change from female to male, the ovary is replaced by the testis through the degeneration of oocytes and subsequent spermatogenesis. In the present study, we cloned a cDNA-encoding gonadal soma-derived factor (GSDF) from protogynous wrasse and examined its expression pattern in the sexually mature gonads and the sex-changing gonad induced experimentally by aromatase inhibition. Results: Expression of gsdf was predominantly observed in the testis, and it was mainly localized to the supporting cells surrounding the spermatogonia. In the ovary, only slight expression of gsdf was observed in morphologically undifferentiated supporting cells in contact with oogonia. During sex change, strong expression of gsdf appeared first in the supporting cells surrounding the gonial germ cells before the onset of spermatogenesis. Thereafter, the expression of gsdf continually increased in the supporting cells surrounding the proliferating spermatogonia throughout the sex change. Conclusions: These results suggest that gsdf is involved in the proliferation of spermatogonia and subsequent spermatogenesis in both the testis and the gonad in the early stages of sex change. Developmental Dynamics 242:388–399, 2013. © 2013 Wiley Periodicals, Inc.

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