In vitro oocyte culture-based manipulation of zebrafish maternal genes


Correspondence to: Francisco Pelegri, Laboratory of Genetics, University of Wisconsin - Madison, 425-G Henry Mall, Madison, WI 53706. E-mail:


Images showing wild type cleavage stage zebrafish embryos (left column), cleavage stage embryos carrying the cei mutation (middle), and cie mutant embryos rescued with wild-type aurB mRNA (right). Green fluorescence visualizes bcatenin, red fluorescence visualizes a-tubulin. Visualization of both channels is seen in the top row. See Nair et al., Developmental Dynamics 242:44–52 for original figure and additional experimental details.