Dact gene expression profiles suggest a role for this gene family in integrating Wnt and TGF-β signaling pathways during chicken limb development

Authors

  • Lucimara Aparecida Sensiate,

    1. Department of Histology and Embryology, State University of Campinas UNICAMP, Campinas, Brazil
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    • Lucimara Aparecida Sensiate and Débora R. Sobreira contributed equally to this work.

  • Débora R. Sobreira,

    1. Department of Histology and Embryology, State University of Campinas UNICAMP, Campinas, Brazil
    2. Institute of Biomedical and Biomolecular Science, University of Portsmouth, Portsmouth, United Kingdom
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    • Lucimara Aparecida Sensiate and Débora R. Sobreira contributed equally to this work.

  • Fernanda Cristina Da Veiga,

    1. Department of Histology and Embryology, State University of Campinas UNICAMP, Campinas, Brazil
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  • Denner Jefferson Peterlini,

    1. Department of Histology and Embryology, State University of Campinas UNICAMP, Campinas, Brazil
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  • Angelica Vasconcelos Pedrosa,

    1. Department of Histology and Embryology, State University of Campinas UNICAMP, Campinas, Brazil
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  • Thaís Rirsch,

    1. Department of Histology and Embryology, State University of Campinas UNICAMP, Campinas, Brazil
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  • Paulo Pinto Joazeiro,

    1. Department of Histology and Embryology, State University of Campinas UNICAMP, Campinas, Brazil
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  • Frank R. Schubert,

    1. Institute of Biomedical and Biomolecular Science, University of Portsmouth, Portsmouth, United Kingdom
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  • Carla Beatriz Collares-Buzato,

    1. Department of Histology and Embryology, State University of Campinas UNICAMP, Campinas, Brazil
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  • José Xavier-Neto,

    1. Laboratório Nacional de Biociências (LNBio), Campinas, Brazil
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  • Susanne Dietrich,

    1. Institute of Biomedical and Biomolecular Science, University of Portsmouth, Portsmouth, United Kingdom
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  • Lúcia Elvira Alvares

    Corresponding author
    1. Department of Histology and Embryology, State University of Campinas UNICAMP, Campinas, Brazil
    • Correspondence to: Lúcia Elvira Alvares, Department of Histology and Embryology, State University of Campinas, UNICAMP, Rua Charles Darwin, s/n° Cx. Postal 6109 CEP 13083-863, Campinas, SP, Brazil. E-mail: lealvare@unicamp.br

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  • This article appeared online February 15, 2013. A production error delayed publication in a monthly issue of the journal.

Abstract

Background: Dact gene family encodes multifunctional proteins that are important modulators of Wnt and TGF-β signaling pathways. Given that these pathways coordinate multiple steps of limb development, we investigated the expression pattern of the two chicken Dact genes (Dact1 and Dact2) from early limb bud up to stages when several tissues are differentiating. Results: During early limb development (HH24-HH30) Dact1 and Dact2 were mainly expressed in the cartilaginous rudiments of the appendicular skeleton and perichondrium, presenting expression profiles related, but distinct. At later stages of development (HH31–HH35), the main sites of Dact1 and Dact2 expression were the developing synovial joints. In this context, Dact1 expression was shown to co-localize with regions enriched in the nuclear β-catenin protein, such as developing joint capsule and interzone. In contrast, Dact2 expression was restricted to the interzone surrounding the domains of bmpR-1b expression, a TGF-β receptor with crucial roles during digit morphogenesis. Additional sites of Dact expression were the developing tendons and digit blastemas. Conclusions: Our data indicate that Dact genes are good candidates to modulate and, possibly, integrate Wnt and TGF-β signaling during limb development, bringing new and interesting perspectives about the roles of Dact molecules in limb birth defects and human diseases. Developmental Dynamics 243:428–439, 2014. © 2013 Wiley Periodicals, Inc.

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