Expression of ski can act as a negative feedback mechanism on retinoic acid signaling
Version of Record online: 9 APR 2013
Copyright © 2013 Wiley Periodicals, Inc.
Volume 242, Issue 6, pages 604–613, June 2013
How to Cite
Melling, M. A., Friendship, C. R.C., Shepherd, T. G. and Drysdale, T. A. (2013), Expression of ski can act as a negative feedback mechanism on retinoic acid signaling. Dev. Dyn., 242: 604–613. doi: 10.1002/dvdy.23954
- Issue online: 20 MAY 2013
- Version of Record online: 9 APR 2013
- Accepted manuscript online: 26 FEB 2013 12:00AM EST
- Manuscript Accepted: 8 FEB 2013
- Manuscript Revised: 23 JAN 2013
- Manuscript Received: 13 SEP 2012
- the Canadian Institutes of Health Research . Grant Number: MOP-74663
- the Canadian Cancer Society . Grant Number: 20109
Additional Supporting Information may be found in the online version of this article.
|dvdy23954-sup-0001-suppfig1.pdf||198K||Supp. Fig. S1. Inhibition of aldh1a2 or cyp26a1 results in changes in ski expression. When embryos were treated with either citral or diethylaminobenzaldehyde (DEAB), levels of both cyp26a1 and ski were reduced. This is seen most clearly in the somites (blue arrows). The in situ hybridization reaction for ski were allowed to fully develop to ensure that the changes were clear, hence, the dark staining. The citral appeared more effective at reducing cyp26a1 and ski expression and resulting in embryos with axial shortening. When cyp26a1 was inhibited by ketaconazole treatment, cyp26a1 expression was increased as expected, best seen in the staining near the trigeminal ganglia (yellow arrows) but we could see no clear increase in ski expression as assayed by in situ hybridization.|
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