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dvdy23954-sup-0001-suppfig1.pdf198KSupp. Fig. S1. Inhibition of aldh1a2 or cyp26a1 results in changes in ski expression. When embryos were treated with either citral or diethylaminobenzaldehyde (DEAB), levels of both cyp26a1 and ski were reduced. This is seen most clearly in the somites (blue arrows). The in situ hybridization reaction for ski were allowed to fully develop to ensure that the changes were clear, hence, the dark staining. The citral appeared more effective at reducing cyp26a1 and ski expression and resulting in embryos with axial shortening. When cyp26a1 was inhibited by ketaconazole treatment, cyp26a1 expression was increased as expected, best seen in the staining near the trigeminal ganglia (yellow arrows) but we could see no clear increase in ski expression as assayed by in situ hybridization.

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