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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
dvdy23961-sup-0001-suppmovie1.mov6605KSupp. Movie S1. Confocal Time-Lapse Movie of Sftpc/GFP lung explants showing the four different stages during bud bifurcation. Lungs were dissected at E11.5, cultured overnight, and thereafter imaged for 970 min. Note that the cleft regions remain largely in place, whereas the bifurcating buds grow out further. Images were taken every 7.5 min. Movie plays at 15 frames per second (fps). Still images from this movie are shown in Figure 1B.
dvdy23961-sup-0002-suppmovie2.mov1321KSupp. Movie S2. Confocal Time-Lapse Movie of H2B/GFP lung explants showing proliferation and spindle reorientation during planar bifurcation. Lungs were dissected at E11.5, cultured overnight, and thereafter imaged for 9.5 hr. Images were taken every 5 min. Red balls indicate proliferating cells throughout the bud and trunk area. Note the change in mitotic chromosome orientation as well as the movement of dividing nuclei into and after division out of the focal plane. Movie plays at 2 fps. Still images from this movie are shown in Figure 2F.
dvdy23961-sup-0003-suppmovie3.mov2178KSupp. Movie S3. Confocal Time-Lapse Movie of H2B/GFP lung explants showing proliferation and spindle reorientation during domain branch formation. Lungs were dissected at E11.5, cultured overnight, and thereafter imaged for 12.5 hr. Images were taken every 5 min. Grey balls indicate proliferating cells throughout the domain branch and trunk area. Movie plays at 15 fps. This movie is related to Figure 3.
dvdy23961-sup-0004-suppmovie4.mov1888KSupp. Movie S4. Confocal Time-Lapse Movie of H2B/GFP lung explants showing individual cell movement during bud bifurcation. Lungs were dissected at E11.5, cultured overnight, and thereafter imaged for 25.5 hr. Images were taken every 6 min. Red squares indicate cell movement behavior over time. Movie plays at 15 fps. Still images from this movie are shown in Figure 5A.
dvdy23961-sup-0005-suppmovie5.mov5167KSupp. Movie S5. Confocal Time-Lapse Movie of H2B/GFP lung explants showing a lack of GFP cell contribution to the formation of a new domain branch. Lungs were dissected at E11.5, cultured overnight, and thereafter imaged for 29.5 hr. Images were taken every 5 min. Movie plays at 15 fps. Still images from this movie are shown in Figure 5B.
dvdy23961-sup-0006-suppmovie6.mov2818KSupp. Movie S6. Confocal Time-Lapse Movie of H2B/GFP lung explants showing individual cell movement during domain branch formation. Lungs were dissected at E11.5, cultured overnight, and thereafter imaged for 12.5 hr. Images were taken every 5 min. Red spheres indicate cell movement behavior over time. Movie plays at 15 fps. Still images from this movie are shown in Figure 5C and D.
dvdy23961-sup-0007-suppmovie7.mov2551KSupp. Movie S7. Confocal Time-Lapse Movie of Control H2B/GFP lung explants treated with DMSO alone. Lungs were dissected at E11.5, cultured overnight, and thereafter imaged for 15 hr in the presence of DMSO. Images were taken every 5 min. Movie plays at 15 fps. Still images from this movie are shown in Figure 8A.
dvdy23961-sup-0008-suppmovie8.mov1496KMovie S8: Confocal Time-Lapse Movie of H2B/GFP lung explants treated with the MLCK inhibitor ML-7 shows a failure in planar bifurcation when buds are at the bud stage (red asterisk) but not after the buds have transitioned to the flattened stage (white asterisk). Lungs were dissected at E11.5, cultured overnight, and thereafter imaged for 16.5 hr in the presence of 5 μM ML-7. Images were taken every 5 min. Movie plays at 15 fps. Still images from this movie are shown in Figure 8B.
dvdy23961-sup-0009-suppmovie9.mov1247KSupp. Movie S9. Confocal Time-Lapse Movie of H2B/GFP lung explants treated with the MLCK inhibitor ML-7 shows no perturbation of domain branch formation (arrow). Lungs were dissected at E11.5, cultured overnight, and thereafter imaged for 16.5 hr in the presence of 5 μM ML-7. Images were taken every 5 min. Movie plays at 15 fps. Still images from this movie are shown in Figure 8C.
dvdy23961-sup-0010-suppmovie10.mov3014KSupp. Movie S10. Confocal Time-Lapse Movie of H2B/GFP lung explants treated with the myosin ATPase inhibitor Blebbistatin shows a general failure in planar bifurcation. Lungs were dissected at E11.5, cultured overnight, and thereafter imaged for 20 hr in the presence of 25 μM Blebbistatin. Images were taken every 5 min. Movie plays at 15 fps. Still images from this movie are shown in Figure 8D.

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