Spatiotemporal expression of zic genes during vertebrate inner ear development

Authors

  • Andrew P. Chervenak,

    1. Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan
    2. Cellular and Molecular Biology Graduate Program, University of Michigan Medical School, Ann Arbor, Michigan
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  • Ibrahim S. Hakim,

    1. Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan
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  • Kate F. Barald

    Corresponding author
    1. Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan
    2. Cellular and Molecular Biology Graduate Program, University of Michigan Medical School, Ann Arbor, Michigan
    3. Department of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan
    • Correspondence to: Kate F. Barald, 3053 BSRB, Department of Cell and Developmental Biology, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200. E-mail: kfbarald@umich.edu

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Abstract

BACKGROUND: Inner ear development involves signaling from surrounding tissues, including the adjacent hindbrain, periotic mesenchyme, and notochord. These signals include SHH, FGFs, BMPs, and WNTs from the hindbrain and SHH from the notochord. Zic genes, which are expressed in the dorsal neural tube and act during neural development, have been implicated as effectors of these pathways. This report examines whether Zic genes' involvement in inner ear development is a tenable hypothesis based on their expression patterns. RESULTS: In the developing inner ear of both the chick and mouse, all of the Zic genes were expressed in the dorsal neural tube and variably in the periotic mesenchyme, but expression of the Zic genes in the otic epithelium was not found. The onset of expression differed among the Zic genes; within any given region surrounding the otic epithelium, multiple Zic genes were expressed in the same place at the same time. CONCLUSIONS: Zic gene expression in the region of the developing inner ear is similar between mouse and chick. Zic expression domains overlap with sites of WNT and SHH signaling during otocyst patterning, suggesting a role for Zic genes in modulating signaling from these pathways. Developmental Dynamics 242:897–908, 2013. © 2013 Wiley Periodicals, Inc.

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